pH-dependent absorption spectra of rhodopsin mutant E113Q: On the role of counterions and protein
文献类型：期刊论文
| 作者 | Alsaedi, Ahmed2; Xie, Peng1; Zhou, Panwang1; Zhang, Yan1 |
| 刊名 | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
 |
| 出版日期 | 2017-03-05 |
| 卷号 | 174页码:25-31 |
| 关键词 | Spectra Tuning Qm/mm E113q Rhodopsin Hydrogen-bond Anion Biosensor |
| ISSN号 | 1386-1425 |
| DOI | 10.1016/j.saa.2016.11.015 |
| 文献子类 | Article |
| 英文摘要 | The absorption spectra of bovine rhodopsin mutant E113Q in solutions were investigated at the molecular level by using a hybrid quantum mechanics/molecular mechanics (QM/MM) method. The calculations suggest the mechanism of the absorption variations of E113Q at different pH values. The results indicate that the polarizations of the counterions in the vicinity of Schiff base under protonation and unprotonation states of the mutant E113Q would be a crucial factor to change the energy gap of the retinal to tune the absorption spectra. Glu-181 residue, which is close to the chromophore, cannot serve as the counterion of the protonated Schiff base of E113Q in dark state. Moreover, the results of the absorption maximum in mutant E113Q with the various anions (Cl-, Br-, I- and NO3-) manifested that the mutant E113Q could have the potential for use as a template of anion biosensors at visible wavelength. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | COUPLED-RECEPTOR RHODOPSIN ; RETINAL CHROMOPHORE ; BOVINE RHODOPSIN ; VISUAL PIGMENTS ; ENERGY-STORAGE ; GLUTAMIC-ACID ; FORCE-FIELDS ; THEORY LEVEL ; SCHIFF-BASE ; STATE |
| WOS研究方向 | Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000393628500004 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169644]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Yan |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Liaoning, Peoples R China 2.King Abdulaziz Univ, Nonlinear Anal & Appl Math NAAM Res Grp, Dept Math, Fac Sci, Jeddah 21589, Saudi Arabia |
| 推荐引用方式 GB/T 7714 | Alsaedi, Ahmed,Xie, Peng,Zhou, Panwang,et al. pH-dependent absorption spectra of rhodopsin mutant E113Q: On the role of counterions and protein[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2017,174:25-31. |
| APA | Alsaedi, Ahmed,Xie, Peng,Zhou, Panwang,&Zhang, Yan.(2017).pH-dependent absorption spectra of rhodopsin mutant E113Q: On the role of counterions and protein.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,174,25-31. |
| MLA | Alsaedi, Ahmed,et al."pH-dependent absorption spectra of rhodopsin mutant E113Q: On the role of counterions and protein".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 174(2017):25-31. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。