Electrospray ionization in concentrated acetonitrile vapor improves the performance of mass spectrometry for proteomic analyses
文献类型：期刊论文
| 作者 | Zou, Hanfa1; Chen, Jin1,2; Wang, Fangjun1; Liu, Zheyi1,2; Liu, Jing1,2; Zhu, Yixin3; Zhang, Yukui1 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2017-02-03 |
| 卷号 | 1483页码:101-109 |
| 关键词 | Acetonitrile Background Interference Mass Spectrometry Electrospray Ionization |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2016.12.075 |
| 文献子类 | Article |
| 英文摘要 | Suppressing the background interferences and enhancing the analytes signals are long-term goals in high performance electrospray ionization mass spectrometry (ESIMS) analyses. We observed that performing electrospray in the presence of a concentrated acetonitrile atmosphere suppresses background interferences and enhances peptide signals. An enclosed nanoESI source was utilized to provide a stable atmosphere of concentrated acetonitrile vapor for high performance ESIMS analyses. The median MS signal intensity increased by 5 times for a set of 23 BSA tryptic peptides in direct ESIMS analysis. Further, the number of reproducibly and precisely quantified peptides could be improved 67% in six replicate label-free quantitative proteome analyses by this strategy. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | GAS-PHASE BASICITIES ; PROTON AFFINITIES ; NANOELECTROSPRAY ; EMITTER ; IDENTIFICATION ; PROTEINS ; SIGNALS ; TRAP |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000393249900013 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169684]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Wang, Fangjun |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Haochuang Biotech Co Ltd, Hangzhou 311121, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zou, Hanfa,Chen, Jin,Wang, Fangjun,et al. Electrospray ionization in concentrated acetonitrile vapor improves the performance of mass spectrometry for proteomic analyses[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1483:101-109. |
| APA | Zou, Hanfa.,Chen, Jin.,Wang, Fangjun.,Liu, Zheyi.,Liu, Jing.,...&Zhang, Yukui.(2017).Electrospray ionization in concentrated acetonitrile vapor improves the performance of mass spectrometry for proteomic analyses.JOURNAL OF CHROMATOGRAPHY A,1483,101-109. |
| MLA | Zou, Hanfa,et al."Electrospray ionization in concentrated acetonitrile vapor improves the performance of mass spectrometry for proteomic analyses".JOURNAL OF CHROMATOGRAPHY A 1483(2017):101-109. |
入库方式： OAI收割
来源：大连化学物理研究所
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