Quantitative secretomic analysis of pancreatic cancer cells in serum-containing conditioned medium
文献类型：期刊论文
| 作者 | Zhang, Lihua2; Liu, Peng1; Weng, Yejing2; Sui, Zhigang2; Wu, Yunhao1; Meng, Xiangli1; Wu, Mengwei1; Jin, Haoyi1; Tan, Xiaodong1; Zhang, Yukui2 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-11-21 |
| 卷号 | 6 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep37606 |
| 文献子类 | Article |
| 英文摘要 | Pancreatic cancer is a highly metastatic and chemo-resistant disease. Secreted proteins involved in cell-cell interactions play an important role in changing the tumor microenvironment. Previous studies generally focus on the secretome of cancer cell line from serum-free media, due to the serious interference of fetal bovine serum (FBS). However, serum-starvation may alter expression patterns of secreted proteins. Hence, efforts to decrease the interference of serum in proteomic analysis of serum-containing media have been hampered to quantitatively measure the tumor secretion levels. Recently, the metabolic labeling, protein equalization, protein fractionation and filter-aided sample preparation (FASP) strategy (MLEFF) has been successfully used to avoid the disturbance of serum on secretome analysis. Here, this efficient method was applied for comparative secretome analysis of two hamster pancreatic cancer cells with differentially metastatic potentials, enabling the observation of 161 differentially expressed proteins, including 106 proteins that had been previously reported and detected in plasma. By integrated analysis of our data and publicly available bioinformatics resources, we found that a combination panel consisting of CDH3, PLAU, and LFNG might improve the prognosis of overall pancreatic cancer survival. These secreted proteins may serve as a potential therapeutic targets for pancreatic cancer metastasis. |
| WOS关键词 | INTEGRATIVE ANALYSIS ; METASTASIS ; INVOLVEMENT ; BIOMARKERS ; ENRICHMENT ; NETWORKS ; PROTEINS ; PROTEOME ; INVASION ; ANTIGEN |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000388082500001 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169724]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Lihua; Tan, Xiaodong |
| 作者单位 | 1.China Med Univ, Shengjing Hosp, Dept Gen Surg 1, Shenyang 110004, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Lihua,Liu, Peng,Weng, Yejing,et al. Quantitative secretomic analysis of pancreatic cancer cells in serum-containing conditioned medium[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Zhang, Lihua.,Liu, Peng.,Weng, Yejing.,Sui, Zhigang.,Wu, Yunhao.,...&Zhang, Yukui.(2016).Quantitative secretomic analysis of pancreatic cancer cells in serum-containing conditioned medium.SCIENTIFIC REPORTS,6. |
| MLA | Zhang, Lihua,et al."Quantitative secretomic analysis of pancreatic cancer cells in serum-containing conditioned medium".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
来源：大连化学物理研究所
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