An improved model for exploring the effect of physicochemical properties of alginate-based microcapsules on their fibrosis formation in vivo
文献类型：期刊论文
| 作者 | Wang, Xiuli3; Ma, Xiaojun2; Zheng, Huizhen2,4; Li, Shen1; Gao, Meng2,4; Ren, Ying2,4; Zheng, Guoshuang2,4; Xie, Hongguo2; Yu, Weiting2 |
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2016 |
| 卷号 | 6期号:96页码:93288-93297 |
| ISSN号 | 2046-2069 |
| DOI | 10.1039/c6ra19294g |
| 文献子类 | Article |
| 英文摘要 | In vivo microcapsule implantation suffers from varying degrees of inflammatory responses. Considering the complications of the implantation process and the individual variation in laboratory animals, a feasible model, constructed in vitro by culturing fibroblasts on the surfaces of different types of microcapsules, is established in the present study. A high consistency is shown between the cell adhesion on beads or microcapsules based on the developed model in vitro and their fibrosis formation after implantation in vivo, which can be used to evaluate the biocompatibility of the microcapsules. Moreover, the relationship between the surface properties of the microcapsules and the cell adhesion behavior was explored using this developed model. It was found that cell adhesion was aggravated with chitosan reaction, but was reduced with alginate neutralization. This was closely related to the variation trend of surface charges, but not surface roughness and the stiffness of alginate-based microcapsules during the process of chitosan coating and alginate neutralization. This model could therefore provide a rapid evaluation and guidance to tailor the optimal surface properties for long-term transplantation. |
| WOS关键词 | CELL-ADHESION ; SUBSTRATE STIFFNESS ; PANCREATIC-ISLETS ; SURFACE ; BIOCOMPATIBILITY ; CAPSULES ; IMMUNOISOLATION ; PROLIFERATION ; ATTACHMENT ; STABILITY |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000385631900008 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169794]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zheng, Huizhen |
| 作者单位 | 1.Dalian Med Univ, Dalian Municipal Cent Hosp, Dept Endocrinol & Metab, Dalian 116033, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 3.Dalian Med Univ, Coll Basic Med Sci, Dept Histol & Embryol, Dalian 116044, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiuli,Ma, Xiaojun,Zheng, Huizhen,et al. An improved model for exploring the effect of physicochemical properties of alginate-based microcapsules on their fibrosis formation in vivo[J]. RSC ADVANCES,2016,6(96):93288-93297. |
| APA | Wang, Xiuli.,Ma, Xiaojun.,Zheng, Huizhen.,Li, Shen.,Gao, Meng.,...&Yu, Weiting.(2016).An improved model for exploring the effect of physicochemical properties of alginate-based microcapsules on their fibrosis formation in vivo.RSC ADVANCES,6(96),93288-93297. |
| MLA | Wang, Xiuli,et al."An improved model for exploring the effect of physicochemical properties of alginate-based microcapsules on their fibrosis formation in vivo".RSC ADVANCES 6.96(2016):93288-93297. |
入库方式： OAI收割
来源：大连化学物理研究所
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