Ionic liquid-based method for direct proteome characterization of velvet antler cartilage
文献类型：期刊论文
| 作者 | Fang, Fei1,2; Sui, Zhigang2; Weng, Yejing1,2; Zhao, Qun2; Deng, Nan3; Zhu, Xudong1,2; Shan, Yichu2; Zhang, Lihua2; Zhang, Yukui2 |
| 刊名 | TALANTA
 |
| 出版日期 | 2016-12-01 |
| 卷号 | 161页码:541-546 |
| 关键词 | Velvet Antler Cartilage Protein Extraction Proteomic Analysis Ionic Liquid [C12-mim]Cl |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2016.08.083 |
| 文献子类 | Article |
| 英文摘要 | The cartilage zone of the velvet antler is richly vascularized, this being a major difference to the classical cartilage, in which there are no blood vessels. Angiogenesis and rapid growth of vasculature in velvet antler cartilage (VAC) make it an ideal model for discovering the novel angiogenic regulatory factors. However, the proteomic analysis of VAC is challenging due to the serious interference of proteoglycans (PGs) and collagens. To achieve a comprehensive proteome characterization of VAC, herein, we developed an ionic liquid-based method using 1-dodecyl-3-methylimidazolium chloride ([C12-mim]Cl) for selective extraction of cellular proteins from VAC. Compared with the previous cetylpyridinium chloride (CPC)-based method, the developed [C12-mim]Cl-based method takes much less processing time, shows facile preparation procedure and good compatibility towards downstream proteomic analysis, leading to the identification of more protein groups (1543 vs 753), membrane proteins (663 vs 279) and trans membrane proteins (217 vs 58). (C) 2004 Published by Elsevier B.V. |
| WOS关键词 | SAMPLE PREPARATION METHOD ; ARTICULAR-CARTILAGE ; CERVUS-ELAPHUS ; TUMOR-GROWTH ; DEER ANTLER ; ANGIOGENESIS ; EXPRESSION ; PROTEOGLYCAN ; MT1-MMP ; BONE |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000386989500069 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169798]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Lihua |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&D Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.CNTC, Zhengzhou Tobacco Res Inst, Zhengzhou 450000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Fei,Sui, Zhigang,Weng, Yejing,et al. Ionic liquid-based method for direct proteome characterization of velvet antler cartilage[J]. TALANTA,2016,161:541-546. |
| APA | Fang, Fei.,Sui, Zhigang.,Weng, Yejing.,Zhao, Qun.,Deng, Nan.,...&Zhang, Yukui.(2016).Ionic liquid-based method for direct proteome characterization of velvet antler cartilage.TALANTA,161,541-546. |
| MLA | Fang, Fei,et al."Ionic liquid-based method for direct proteome characterization of velvet antler cartilage".TALANTA 161(2016):541-546. |
入库方式： OAI收割
来源：大连化学物理研究所
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