A liquid crystal-based sensor for the simple and sensitive detection of cellulase and cysteine
文献类型：期刊论文
| 作者 | Gao, Yan'an4; Hu, Qiongzheng2; Tian, Tongtong1,3; Yu, Li1; Wang, Yi1 |
| 刊名 | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| 出版日期 | 2016-11-01 |
| 卷号 | 147页码:100-105 |
| 关键词 | Liquid Crystals Sensor Cellulase Cysteine |
| ISSN号 | 0927-7765 |
| DOI | 10.1016/j.colsurfb.2016.07.059 |
| 文献子类 | Article |
| 英文摘要 | A liquid crystal (LC)-based sensor, which is capable of monitoring enzymatic activity at the aqueous/LC interface and detecting cellulase and cysteine (Cys), was herein reported. When functionalized with a surfactant, dodecyl beta-D-glucopyranosicie, the 4-cyano-4'-pentylbiphenyl (5CB) displays a dark-to-bright transition in the optical appearance for cellulase. We attribute this change to the orientational transition of LCs, as a result of enzymatic hydrolysis between cellulase and surfactant. Furthermore, by adding cellulase and Cu2+, our surfactant-LCs system performs an interesting ability to detect Cys, even though Cys could not interact with surfactant or LC directly. Alternatively, through the strong binding between Cys and Cu2+, cellulase was able to hydrolyze surfactant in the presence of Cu2+, leading to the transition of LCs from dark to bright. The detection limit of the LC sensor was around 1 x 10(-5) mg/mL and 82.5 mu M for cellulase and Cys, respectively. The LC-based sensor may contribute to the development of low-cost, expedient, and label-free detection for cellulase and Cys and the design strategy may also provide a novel way for detecting multiple analytes. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | HEAVY-METALS ; NANOCLUSTERS ; POLYELECTROLYTE ; HOMOCYSTEINE ; BIOSENSORS ; SUBSTRATE ; PROTEINS ; SURFACES ; DROPLETS ; PHENOLS |
| WOS研究方向 | Biophysics ; Chemistry ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000384851400012 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169846]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Yu, Li |
| 作者单位 | 1.Shandong Univ, Minist Educ, Key Lab Colloid & Interface Chem, Jinan 250100, Peoples R China 2.Univ Houston, Dept Chem, Houston, TX 77204 USA 3.Qufu Normal Univ, Sch Chem & Chem Engn, Qufu 273165, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Yan'an,Hu, Qiongzheng,Tian, Tongtong,et al. A liquid crystal-based sensor for the simple and sensitive detection of cellulase and cysteine[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2016,147:100-105. |
| APA | Gao, Yan'an,Hu, Qiongzheng,Tian, Tongtong,Yu, Li,&Wang, Yi.(2016).A liquid crystal-based sensor for the simple and sensitive detection of cellulase and cysteine.COLLOIDS AND SURFACES B-BIOINTERFACES,147,100-105. |
| MLA | Gao, Yan'an,et al."A liquid crystal-based sensor for the simple and sensitive detection of cellulase and cysteine".COLLOIDS AND SURFACES B-BIOINTERFACES 147(2016):100-105. |
入库方式： OAI收割
来源：大连化学物理研究所
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