Rational Design of a Profluorescent Substrate for S-adenosylhomocysteine Hydrolase and its Applications in Bioimaging and Inhibitor Screening
文献类型：期刊论文
| 作者 | Zhao, Guangjiu1; Zheng, Daoyuan1; Li, Dongmei4; Wang, Jiayue1; Wang, Yanni1,2; Li, Chunyan1; Han, Keli1; Jia, Yan1,2; Li, Peng1; Song, Wei3 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2016-10-05 |
| 卷号 | 8期号:39页码:25818-25824 |
| 关键词 | S-adenosylhomocysteine Hydrolase Fluorogenic Substrate Kinetic Study Bioimaging Inhibitor Screening |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.6b09190 |
| 文献子类 | Article |
| 英文摘要 | S-Adenosylhomocysteine hydrolase (SAHase) is a cellular enzyme that plays a key role in the methylation process, and a potential drug target in the discovery of antiviral and anticancer agents. There is increasing interest in determining its activity in the biological and clinical fields with chemosensors but with limited success so far. Herein, we designed and developed for the first time an off/on-type of fluorogenic substrate (NADE) that is directly responsive to SAHase activity. NADE used 1,8-naphthalimide as the signal reporter and adenosine (Ade) as the reaction center; removal of the Ade moiety enhanced the fluorescence by >10-fold. Kinetic study showed that NADE followed a non-Michaelis-Menten pattern that corresponded to the allosteric behavior of SAHase. NADE showed excellent selectivity and functioned efficiently in cells, allowing the microscopic imaging of SAHase activity. NADE can also be used to identify and measure the effectiveness of inhibitors in a markedly superior way. In a word, NADE would be broadly useful in clinical applications and academic studies. |
| WOS关键词 | ADENOSYL-L-HOMOCYSTEINE ; COLORIMETRIC ASSAY ; METHIONINE CYCLE ; ANTIVIRAL AGENTS ; ANALOGS ; DEFICIENCY ; DENSITY ; MECHANISM ; CELLS ; ADENOSYLMETHIONINE |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000384951800020 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169884]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Han, Keli |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Dalian Med Univ, Affiliated Hosp 1, Dalian 116023, Peoples R China 4.Nankai Univ, Coll Pharm, Haihe Educ Pk,38 Tongyan Rd, Tianjin 300353, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Guangjiu,Zheng, Daoyuan,Li, Dongmei,et al. Rational Design of a Profluorescent Substrate for S-adenosylhomocysteine Hydrolase and its Applications in Bioimaging and Inhibitor Screening[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(39):25818-25824. |
| APA | Zhao, Guangjiu.,Zheng, Daoyuan.,Li, Dongmei.,Wang, Jiayue.,Wang, Yanni.,...&Song, Wei.(2016).Rational Design of a Profluorescent Substrate for S-adenosylhomocysteine Hydrolase and its Applications in Bioimaging and Inhibitor Screening.ACS APPLIED MATERIALS & INTERFACES,8(39),25818-25824. |
| MLA | Zhao, Guangjiu,et al."Rational Design of a Profluorescent Substrate for S-adenosylhomocysteine Hydrolase and its Applications in Bioimaging and Inhibitor Screening".ACS APPLIED MATERIALS & INTERFACES 8.39(2016):25818-25824. |
入库方式： OAI收割
来源：大连化学物理研究所
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