Rapid and Sensitive Differentiating Ischemic and Hemorrhagic Strokes by Dried Blood Spot Based Direct Injection Mass Spectrometry Metabolomics Analysis
文献类型：期刊论文
| 作者 | Fang, Yanhua6,7; Gao, Peng1,3,5,6,7; Sun Hongzhi5,6,7; Hu, Zhansheng5; Zhu, Zhitu5; Cao, Yunfeng1,5,6,7; Wang, Lixuan6,7; Sun, Xiaoyu6,7; Dong, Jun6,7; Fang, Zhongze2,4,6,7 |
| 刊名 | JOURNAL OF CLINICAL LABORATORY ANALYSIS
 |
| 出版日期 | 2016-11-01 |
| 卷号 | 30期号:6页码:823-830 |
| 关键词 | Stroke Mass Spectrometry Metabolomics |
| ISSN号 | 0887-8013 |
| DOI | 10.1002/jcla.21943 |
| 文献子类 | Article |
| 英文摘要 | Cerebral infarction (CI) and intracerebral hemorrhage are lethal cerebrovascular diseases, sometimes sharing similar clinical manifestations but with distinct therapeutic strategies. Delayed treatment usually resulted in poor prognosis. A timely diagnosis report is highly warranted especially in emergency. One hundred twenty-nine CI patients, 73 intracerebral hemorrhage (ICH) patients, and 98 controls were enrolled in this study. A direct injection mass spectrometry metabolomics approach was adopted using dried blood spot samples. This targeted metabolomics analysis focused on absolute quantitation of 23 amino acids, 26 carnitine/carnitine esters, and 22 calculated ratios parameters. Compared to the normal control group, CI and ICH showed distinct metabolite changes, respectively. For stroke differentiation, Tyr, C5-OH/C0, Cit, Asn, Pro, Val, Arg/Orn, Leu, and Val/Phe were elevated in the CI group. On the contrary, C5:1, Phe/Tyr, (C0 + C2 + C3 + C16 + C18:1)/Cit, and Met/Leu were of lower levels in the CI group. Using regression model based on some of the above-mentioned parameters, 79.07% of stroke patients from a new set could be definitely confirmed. This study proved the targeted metabolomics analysis was a promising tool for rapid and timely stroke differentiation. |
| WOS关键词 | REGIONAL BURDEN ; GLOBAL BURDEN ; BRAIN-DAMAGE ; LC-MS/MS ; DISEASE ; METABONOMICS |
| WOS研究方向 | Medical Laboratory Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000386935200005 |
| 出版者 | JOHN WILEY & SONS INC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169902]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Sun Hongzhi |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 2.Tianjin Med Univ, Tianjin Life Sci Res Ctr, Tianjin, Peoples R China 3.Dalian Sixth Peoples Hosp, Clin Lab, Dalian, PR, Peoples R China 4.Tianjin Med Univ, Dept Microbiol, Sch Basic Med Sci, Tianjin, Peoples R China 5.Liaoning Med Univ, Affiliated Hosp 1, Jinzhou 121000, Peoples R China 6.Chinese Acad Sci, Joint Ctr Translat Med, Dalian Inst Chem Phys, Dalian, Peoples R China 7.Liaoning Med Univ, Affiliated Hosp 1, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Yanhua,Gao, Peng,Sun Hongzhi,et al. Rapid and Sensitive Differentiating Ischemic and Hemorrhagic Strokes by Dried Blood Spot Based Direct Injection Mass Spectrometry Metabolomics Analysis[J]. JOURNAL OF CLINICAL LABORATORY ANALYSIS,2016,30(6):823-830. |
| APA | Fang, Yanhua.,Gao, Peng.,Sun Hongzhi.,Hu, Zhansheng.,Zhu, Zhitu.,...&Xu, Xiaoxue.(2016).Rapid and Sensitive Differentiating Ischemic and Hemorrhagic Strokes by Dried Blood Spot Based Direct Injection Mass Spectrometry Metabolomics Analysis.JOURNAL OF CLINICAL LABORATORY ANALYSIS,30(6),823-830. |
| MLA | Fang, Yanhua,et al."Rapid and Sensitive Differentiating Ischemic and Hemorrhagic Strokes by Dried Blood Spot Based Direct Injection Mass Spectrometry Metabolomics Analysis".JOURNAL OF CLINICAL LABORATORY ANALYSIS 30.6(2016):823-830. |
入库方式： OAI收割
来源：大连化学物理研究所
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