Application of LC-MS-based metabolomics method in differentiating septic survivors from non-survivors
文献类型：期刊论文
| 作者 | Amathieu, Roland2,3; Xu, Guowang1; Liu, Zhicheng1,2; Yin, Peiyuan1; Savarin, Philippe2 |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2016-11-01 |
| 卷号 | 408期号:27页码:7641-7649 |
| 关键词 | Septic Shock Liquid Chromatography-mass Spectrometry Metabolomics Early Mortality Catabolism |
| ISSN号 | 1618-2642 |
| DOI | 10.1007/s00216-016-9845-9 |
| 文献子类 | Article |
| 英文摘要 | Septic shock is the most severe form of sepsis, which is still one of the leading causes of death in the intensive care unit (ICU). Even though early prognosis and diagnosis are known to be indispensable for reaching an optimistic outcome, pathogenic complexities and the lack of specific treatment make it difficult to predict the outcome individually. In the present study, serum samples from surviving and non-surviving septic shock patients were drawn before clinical intervention at admission. Metabolic profiles of all the samples were analyzed by liquid chromatography-mass spectrometry (LC-MS)-based metabolomics. One thousand four hundred nineteen peaks in positive mode and 1878 peaks in negative mode were retained with their relative standard deviation (RSD) below 30 %, in which 187 metabolites were initially identified by retention time and database in the light of the exact molecular mass. Differences between samples from the survivors and the non-survivors were investigated using multivariate and univariate analysis. Finally, 43 significantly varied metabolites were found in the comparison between survivors and non-survivors. Concretely, metabolites in the tricarboxylic acid (TCA) cycle, amino acids, and several energy metabolism-related metabolites were up-regulated in the non-survivors, whereas those in the urea cycle and fatty acids were generally down-regulated. Metabolites such as lysine, alanine, and methionine did not present significant changes in the comparison. Six metabolites were further defined as primary discriminators differentiating the survivors from the non-survivors at the early stage of septic shock. Our findings reveal that LC-MS-based metabolomics is a useful tool for studying septic shock. |
| WOS关键词 | EARLY PROGNOSTIC EVALUATION ; FATTY-ACID OXIDATION ; EXPERIMENTAL SEPSIS ; HEPATOCELLULAR-CARCINOMA ; METABONOMIC APPROACH ; NEONATAL SEPSIS ; SHOCK ; OUTCOMES ; MASS ; ACCUMULATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000386061300014 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169954]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Xu, Guowang |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China 2.Univ Paris 13, Sorbonne Paris Cite, UMR 7244, Lab Chim Struct & Proprietes Biomat & Agents Ther, Rue Chablis 1, F-93000 Bobigny, France 3.Jean Verdier Teaching Hosp, AP HP, Intens Care Unit, F-93140 Bondy, France |
| 推荐引用方式 GB/T 7714 | Amathieu, Roland,Xu, Guowang,Liu, Zhicheng,et al. Application of LC-MS-based metabolomics method in differentiating septic survivors from non-survivors[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2016,408(27):7641-7649. |
| APA | Amathieu, Roland,Xu, Guowang,Liu, Zhicheng,Yin, Peiyuan,&Savarin, Philippe.(2016).Application of LC-MS-based metabolomics method in differentiating septic survivors from non-survivors.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,408(27),7641-7649. |
| MLA | Amathieu, Roland,et al."Application of LC-MS-based metabolomics method in differentiating septic survivors from non-survivors".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 408.27(2016):7641-7649. |
入库方式： OAI收割
来源：大连化学物理研究所
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