A highly selective hydrophilic sorbent for enrichment of N-linked glycopeptides
文献类型：期刊论文
| 作者 | Fu, Dongmei2; Peng, Yahui1; Zhang, Feifang1; Yang, Bingcheng1; Yu, Long2; Liang, Xinmiao1,2 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2016-08-19 |
| 卷号 | 1460页码:197-201 |
| 关键词 | Glycopeptides Hydrophilic Interaction Chromatography Hyperbranched Polyethylenimine Solid Phase Extraction |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2016.07.028 |
| 文献子类 | Article |
| 英文摘要 | Selective enrichment and purification of N-glycopeptides from complex biological samples is often a necessary step for effective identification of low abundance glycopeptides. A polyethylenimine functionalized sorbent described here demonstrates good enrichment ability and excellent selectivity towards acidic glycopeptides (e.g. sialylated glycopeptides), even at 1:3000 mass ratio of target glycopeptides and non-glycopeptides, which is much more superior to commercial ZIC-HILIC and other reported sorbents. It also shows effective enrichment for neutral glycopeptides (IgG). Another desirable feature of such sorbent is its large binding capacity (similar to 220 mg/g for fetuin). (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | INTERACTION LIQUID-CHROMATOGRAPHY ; FUNCTIONALIZED STATIONARY-PHASE ; LECTIN AFFINITY-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; EXCHANGE CHROMATOGRAPHY ; EXTRACTION ; GLYCOPROTEINS ; MALTOSE ; SILICA ; MATRIX |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000381536400023 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170040]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Yang, Bingcheng |
| 作者单位 | 1.East China Univ Sci & Technol, Sch Pharm, 130 Meilong RD, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Dongmei,Peng, Yahui,Zhang, Feifang,et al. A highly selective hydrophilic sorbent for enrichment of N-linked glycopeptides[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1460:197-201. |
| APA | Fu, Dongmei,Peng, Yahui,Zhang, Feifang,Yang, Bingcheng,Yu, Long,&Liang, Xinmiao.(2016).A highly selective hydrophilic sorbent for enrichment of N-linked glycopeptides.JOURNAL OF CHROMATOGRAPHY A,1460,197-201. |
| MLA | Fu, Dongmei,et al."A highly selective hydrophilic sorbent for enrichment of N-linked glycopeptides".JOURNAL OF CHROMATOGRAPHY A 1460(2016):197-201. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。