Dipeptide-Based Carbohydrate Receptors and Polymers for Glycopeptide Enrichment and Glycan Discrimination
文献类型：期刊论文
| 作者 | Qing, Guangyan1; Li, Xiuling2; Xiong, Peng1; Chen, Cheng2; Zhan, Mimi1; Liang, Xinmiao2; Sun, Taolei1,3 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2016-08-31 |
| 卷号 | 8期号:34页码:22084-22092 |
| 关键词 | Polymer Interface Glycoproteomics Carbohydrate Enrichment |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.6b07863 |
| 文献子类 | Article |
| 英文摘要 | Glycoproteomics identifies and catalogs protein glycosylation and explores its impact on protein conformations and biofunctions. However, these studies are restricted by the bottleneck to enrich low-abundance glycopeptides from complex biosamples and the difficulties in analyzing glycan structures by mass spectrometry. Here, we report dipeptide as a simple but promising carbohydrate binding platform to tackle these problems. We build a hydropathy-index-based strategy for sequence optimization and screen out three optimal dipeptide sequences from 54 types of dipeptides. The optimized dipeptide-based homopolymers display excellent performance (e.g., selectivity up to similar to 70% for real biosamples and strong anti-interference capacity capable of resisting 1000-fold bovine serum albumin interference) glycopeptide enrichment. Meanwhile, our polymers exhibit high-efficiency chromatographic separation toward oligosaccharides with different compositions, polymerization degrees and even their linkage isomers. This brings another attractive feature that our materials can discriminate subtly variable glycan structures of glycopeptides, especially, isomeric glycosidic linkages. These features provide a solid foundation to analyze the complex glycan structures and glycosites simultaneously, which will benefit future development of glycoproteomics and glycobiology. |
| WOS关键词 | HYDROPHILIC INTERACTION CHROMATOGRAPHY ; TITANIUM-DIOXIDE CHROMATOGRAPHY ; PROTEIN GLYCOSYLATION ANALYSIS ; MASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; SELECTIVE ENRICHMENT ; HYDRAZIDE CHEMISTRY ; CANCER ; IDENTIFICATION ; RECOGNITION |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000382514100029 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170126]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Liang, Xinmiao; Sun, Taolei |
| 作者单位 | 1.Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, 122 Luoshi Rd, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China 3.Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, 122 Luoshi Rd, Wuhan 430070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qing, Guangyan,Li, Xiuling,Xiong, Peng,et al. Dipeptide-Based Carbohydrate Receptors and Polymers for Glycopeptide Enrichment and Glycan Discrimination[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(34):22084-22092. |
| APA | Qing, Guangyan.,Li, Xiuling.,Xiong, Peng.,Chen, Cheng.,Zhan, Mimi.,...&Sun, Taolei.(2016).Dipeptide-Based Carbohydrate Receptors and Polymers for Glycopeptide Enrichment and Glycan Discrimination.ACS APPLIED MATERIALS & INTERFACES,8(34),22084-22092. |
| MLA | Qing, Guangyan,et al."Dipeptide-Based Carbohydrate Receptors and Polymers for Glycopeptide Enrichment and Glycan Discrimination".ACS APPLIED MATERIALS & INTERFACES 8.34(2016):22084-22092. |
入库方式： OAI收割
来源：大连化学物理研究所
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