A nonionic surfactant-decorated liquid crystal sensor for sensitive and selective detection of proteins
文献类型：期刊论文
| 作者 | Wang, Yi2; Hu, Qiongzheng3; Tian, Tongtong1,2; Gao, Yan'an4; Yu, Li2 |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2016-09-21 |
| 卷号 | 937页码:119-126 |
| 关键词 | Liquid Crystals Sensor Nonionic Surfactant Lysozyme Concanavalin a Bovine Serum Albumin |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2016.07.010 |
| 文献子类 | Article |
| 英文摘要 | Proteins are responsible for most biochemical events in human body. It is essential to develop sensitive and selective methods for the detection of proteins. In this study, liquid crystal (LC)-based sensor for highly selective and sensitive detection of lysozyme, concanavalin A (Con A), and bovine serum albumin (BSA) was constructed by utilizing the LC interface decorated with a nonionic surfactant, dodecyl beta-Dglucopyranoside. A change of the LC optical images from bright to dark appearance was observed after transferring dodecyl beta-D-glucopyranoside onto the aqueous/LC interface due to the formation of stable self-assembled surfactant monolayer, regardless of pH and ion concentrations studied in a wide range. The optical images turned back from dark to bright appearance after addition of lysozyme, Con A and BSA, respectively. Noteworthy is that these proteins can be further distinguished by adding enzyme inhibitors and controlling incubation temperature of the protein solutions based on three different interaction mechanisms between proteins and dodecyl beta-D-glucopyranoside, viz. enzymatic hydrolysis, specific saccharide binding, and physical absorption. The LC-based sensor decorated with dodecyl beta-Dglucopyranoside shows high sensitivity for protein detection. The limit of detection (LOD) for lysozyme, Con A and BSA reaches around 0.1 mg/mL, 0.01 mg/mL and 0.001 mg/mL, respectively. These results might provide new insights into increasing selectivity and sensitivity of LC-based sensors for the detection of proteins. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | INDOLE-DERIVATIVES ; LYSOZYME ; INTERFACES ; BINDING ; INHIBITION ; LIPOPOLYSACCHARIDE ; FLUORESCENCE ; ADSORPTION ; IMIDAZOLE ; BIOSENSOR |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000382252800015 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170132]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Yu, Li |
| 作者单位 | 1.Qufu Normal Univ, Sch Chem & Chem Engn, Qufu 273165, Peoples R China 2.Shandong Univ, Minist Educ, Key Lab Colloid & Interface Chem, Jinan 250100, Peoples R China 3.Univ Houston, Dept Chem, Houston, TX 77204 USA 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yi,Hu, Qiongzheng,Tian, Tongtong,et al. A nonionic surfactant-decorated liquid crystal sensor for sensitive and selective detection of proteins[J]. ANALYTICA CHIMICA ACTA,2016,937:119-126. |
| APA | Wang, Yi,Hu, Qiongzheng,Tian, Tongtong,Gao, Yan'an,&Yu, Li.(2016).A nonionic surfactant-decorated liquid crystal sensor for sensitive and selective detection of proteins.ANALYTICA CHIMICA ACTA,937,119-126. |
| MLA | Wang, Yi,et al."A nonionic surfactant-decorated liquid crystal sensor for sensitive and selective detection of proteins".ANALYTICA CHIMICA ACTA 937(2016):119-126. |
入库方式： OAI收割
来源：大连化学物理研究所
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