Graphene oxide-based boronate polymer brushes via surface initiated atom transfer radical polymerization for the selective enrichment of glycoproteins
文献类型：期刊论文
| 作者 | An, Xiangyang1; He, Xiwen1; Chen, Langxing1,2; Zhang, Yukui1,3 |
| 刊名 | JOURNAL OF MATERIALS CHEMISTRY B
 |
| 出版日期 | 2016 |
| 卷号 | 4期号:36页码:6125-6133 |
| ISSN号 | 2050-750X |
| DOI | 10.1039/c6tb01489e |
| 文献子类 | Article |
| 英文摘要 | Biomedical sciences, and in particular biomarker research, demand efficient glycoprotein enrichment platforms. In this work, a facile and efficient method was developed to synthesize boronic acid polymer brushes immobilized on magnetic graphene oxide via surface initiated atom transfer radical polymerization (SI-ATRP) for the selective enrichment of glycoproteins from complex biological samples. The magnetic graphene oxide (GO@Fe3O4) nanocomposites were prepared by a solvothermal reaction, providing an ultrahigh surface area and allowing fast separation. Through the self-assembly procedure, the pyrene-based initiators (GO@Br) of SI-ATRP were easily functionalized on the GO sheet via noncovalent pi-pi interaction between pyrene and GO. Finally, the well-defined and high density poly(4-vinylphenylboronic acid) brushes (GO@PVPBA) via SI-ATRP were successfully fabricated. The morphology and structure of GO@Fe(3)O4, GO@Br, and GO@PVPBA nanocomposites were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), vibrating sample magnetometry (VSM), Fourier transform-infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). The selective recognition capability of GO@PVPBA nanocomposites was demonstrated by the selective enrichment of glycoproteins from a complex system consisting of standard proteins ovalbumin (OVA), transferrin (Trf), bovine serum albumin (BSA), and lysozyme (Lyz). Furthermore, the GO@PVPBA nanocomposite also exhibited a high binding capacity up to 514.8 and 445.9 mg g(-1) for OVA and Trf, respectively, and was applied to capture directly glycoproteins from the egg white samples. |
| WOS关键词 | FUNCTIONALIZED MAGNETIC NANOPARTICLES ; AFFINITY MONOLITHIC COLUMN ; EFFECTIVE ADSORBENTS ; TRACE GLYCOPROTEINS ; FACILE SYNTHESIS ; CIS-DIOL ; CAPTURE ; RECOGNITION ; COMPOSITE ; GLYCOPEPTIDES |
| WOS研究方向 | Materials Science |
| 语种 | 英语 |
| 出版者 | ROYAL SOC CHEMISTRY |
| WOS记录号 | WOS:000384233900018 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170136]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Chen, Langxing; Zhang, Yukui |
| 作者单位 | 1.Nankai Univ, Res Ctr Analyt Sci, Coll Chem, Tianjin Key Lab Biosensing & Mol Recognit,State K, Tianjin 300071, Peoples R China 2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | An, Xiangyang,He, Xiwen,Chen, Langxing,et al. Graphene oxide-based boronate polymer brushes via surface initiated atom transfer radical polymerization for the selective enrichment of glycoproteins[J]. JOURNAL OF MATERIALS CHEMISTRY B,2016,4(36):6125-6133. |
| APA | An, Xiangyang,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2016).Graphene oxide-based boronate polymer brushes via surface initiated atom transfer radical polymerization for the selective enrichment of glycoproteins.JOURNAL OF MATERIALS CHEMISTRY B,4(36),6125-6133. |
| MLA | An, Xiangyang,et al."Graphene oxide-based boronate polymer brushes via surface initiated atom transfer radical polymerization for the selective enrichment of glycoproteins".JOURNAL OF MATERIALS CHEMISTRY B 4.36(2016):6125-6133. |
入库方式： OAI收割
来源：大连化学物理研究所
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