Hydrophobic Tagging-Assisted N-Termini Enrichment for In-Depth N-Terminome Analysis
文献类型：期刊论文
| 作者 | Zhang, Lihua1; Chen, Lingfan1,2; Shan, Yichu1; Weng, Yejing1,2; Sui, Zhigang1; Zhang, Xiaodan1; Liang, Zhen1; Zhang, Yukui1 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2016-09-06 |
| 卷号 | 88期号:17页码:8390-8395 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.6b02453 |
| 文献子类 | Article |
| 英文摘要 | The analysis of protein N-termini is of great importance for understanding the protein function and elucidating the proteolytic processing. Herein, we develop a negative enrichment strategy, termed as hydrophobic tagging-assisted N-termini enrichment (HYTANE) to achieve a global N-terminome analysis. The HYTANE strategy showed a high efficiency in hydrophobic tagging and C18 material-assisted depletion using bovine serum albumin (BSA) as the sample. This strategy was applied to N-termini profiling from S. cerevisiae cell lysates and enabled the identification of 1096 protein N-termini, representing the largest N-terminome data set of S. cerevisiae. The identified N-terminal peptides accounted for 99% of all identified peptides, and no deficiency in acidic, histidine (His)-containing, and His-free N-terminal peptides was observed. The presented HYTANE strategy is therefore a highly selective, efficient, and unbiased strategy for the large scale N-terminome analysis. Furthermore, using the HYTANE strategy, we identified 329 cleavage sites and 291 substrates of caspases in Jurkat cells, demonstrating the great promise of HYTANE strategy for protease research. Data are available via ProteomeXchange with identifier PXD004690. |
| WOS关键词 | POSITIONAL PROTEOMICS ; SELECTIVE ISOLATION ; MASS-SPECTROMETRY ; CLEAVAGE SITES ; PEPTIDES ; PROTEINS ; ACETYLATION ; COMPLEX ; APOPTOSIS ; STRATEGY |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000382805900010 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170254]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Lihua |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Lihua,Chen, Lingfan,Shan, Yichu,et al. Hydrophobic Tagging-Assisted N-Termini Enrichment for In-Depth N-Terminome Analysis[J]. ANALYTICAL CHEMISTRY,2016,88(17):8390-8395. |
| APA | Zhang, Lihua.,Chen, Lingfan.,Shan, Yichu.,Weng, Yejing.,Sui, Zhigang.,...&Zhang, Yukui.(2016).Hydrophobic Tagging-Assisted N-Termini Enrichment for In-Depth N-Terminome Analysis.ANALYTICAL CHEMISTRY,88(17),8390-8395. |
| MLA | Zhang, Lihua,et al."Hydrophobic Tagging-Assisted N-Termini Enrichment for In-Depth N-Terminome Analysis".ANALYTICAL CHEMISTRY 88.17(2016):8390-8395. |
入库方式： OAI收割
来源：大连化学物理研究所
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