Microfabrication of a tunable collagen/alginate-chitosan hydrogel membrane for controlling cell-cell interactions
文献类型：期刊论文
| 作者 | Song, Yizhe1,2; Zhang, Demeng1,2; Lv, Yan1,2; Guo, Xin2; Lou, Ruyun1,2; Wang, Shujun2; Wang, Xiuli3; Yu, Weiting2; Ma, Xiaojun2 |
| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2016-11-20 |
| 卷号 | 153页码:652-662 |
| 关键词 | Collagen/alginate-chitosan Hydrogel Membrane Indirect Cell Contact Co-culture Cell-cell Interactions Intercellular Distance |
| ISSN号 | 0144-8617 |
| DOI | 10.1016/j.carbpol.2016.07.058 |
| 文献子类 | Article |
| 英文摘要 | Indirect cell contact co-culture system is increasingly becoming more attractable owing to their advantages of easy cell separation and desirable outcomes for cell-cell interactions. However, how to precisely control the spatial position of cells within multicellular co-cultures is still experimentally challenging due to the incapability of the conventional methods in vitro. In the present study, a tunable collagen/alginatechitosan (Col/Alg-Chi) membrane was established, which was capable of controlling intercellular distance between the neighboring cells at a level of micrometer resolution. It was showed that intercellular distance between the hepatocytes and the fibroblasts exerted significant influence on hepatic function in vitro. In particular, maintenance of the functionality of primary hepatocytes requires direct contact between the hepatocytes and their supportive stromal cells, and their effective contact distance is within 30 mu m. This technical platform would potentially enable investigations of dynamic cell-cell interaction in a multitude of applications including organogenesis, development or even neoplastic transformation. (C) 2016 Published by Elsevier Ltd. |
| WOS关键词 | EMBRYONIC STEM-CELLS ; EX-VIVO EXPANSION ; IN-VITRO ; POROUS MEMBRANES ; COCULTURE SYSTEM ; HEPATOCYTE ; CULTURE ; 3D ; DIFFERENTIATION ; GROWTH |
| WOS研究方向 | Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000382317200073 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170278]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Wang, Xiuli; Yu, Weiting |
| 作者单位 | 1.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, 457 Zhongshan Rd, Dalian 116023, Peoples R China 3.Dalian Med Univ, Coll Basic Med Sci, Dept Histol & Embryol, Dalian 116044, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Yizhe,Zhang, Demeng,Lv, Yan,et al. Microfabrication of a tunable collagen/alginate-chitosan hydrogel membrane for controlling cell-cell interactions[J]. CARBOHYDRATE POLYMERS,2016,153:652-662. |
| APA | Song, Yizhe.,Zhang, Demeng.,Lv, Yan.,Guo, Xin.,Lou, Ruyun.,...&Ma, Xiaojun.(2016).Microfabrication of a tunable collagen/alginate-chitosan hydrogel membrane for controlling cell-cell interactions.CARBOHYDRATE POLYMERS,153,652-662. |
| MLA | Song, Yizhe,et al."Microfabrication of a tunable collagen/alginate-chitosan hydrogel membrane for controlling cell-cell interactions".CARBOHYDRATE POLYMERS 153(2016):652-662. |
入库方式： OAI收割
来源：大连化学物理研究所
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