Development of a Functional Glomerulus at the Organ Level on a Chip to Mimic Hypertensive Nephropathy
文献类型：期刊论文
| 作者 | Zhou, Mengying2; Zhang, Xulang1; Wen, Xinyu2; Wu, Taihua2; Wang, Weidong2; Yang, Mingzhou3; Wang, Jing2; Fang, Ming2; Lin, Bingcheng1; Lin, Hongli2 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-08-25 |
| 卷号 | 6 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep31771 |
| 文献子类 | Article |
| 英文摘要 | Glomerular hypertension is an important factor exacerbating glomerular diseases to end-stage renal diseases because, ultimately, it results in glomerular sclerosis (especially in hypertensive and diabetic nephropathy). The precise mechanism of glomerular sclerosis caused by glomerular hypertension is unclear, due partly to the absence of suitable in vitro or in vivo models capable of mimicking and regulating the complex mechanical forces and/or organ-level disease processes. We developed a "glomerulus-on-a-chip" (GC) microfluidic device. This device reconstitutes the glomerulus with organ-level glomerular functions to create a disease model-on-a chip that mimics hypertensive nephropathy in humans. It comprises two channels lined by closely opposed layers of glomerular endothelial cells and podocytes that experience fluid flow of physiological conditions to mimic the glomerular microenvironment in vivo. Our results revealed that glomerular mechanical forces have a crucial role in cellular cytoskeletal rearrangement as well as the damage to cells and their junctions that leads to increased glomerular leakage observed in hypertensive nephropathy. Results also showed that the GC could readily and flexibly meet the demands of a renal-disease model. The GC could provide drug screening and toxicology testing, and create potential new personalized and accurate therapeutic platforms for glomerular disease. |
| WOS关键词 | ON-A-CHIP ; SLIT DIAPHRAGM ; SHEAR-STRESS ; CELL ADHESION ; PODOCYTES ; MICROFLUIDICS ; BIOLOGY ; CULTURE ; INDUCTION ; APOPTOSIS |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000381883200002 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170294]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Lin, Bingcheng; Lin, Hongli |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 1, Dept Nephrol, Key Lab Kidney Dis Liaoning Prov,Ctr Transformat, 222 Zhongshan Rd, Dalian 116011, Peoples R China 3.Dalian Friendship Hosp, Dept Urol, 8 Sanba Sq, Dalian 116001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Mengying,Zhang, Xulang,Wen, Xinyu,et al. Development of a Functional Glomerulus at the Organ Level on a Chip to Mimic Hypertensive Nephropathy[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Zhou, Mengying.,Zhang, Xulang.,Wen, Xinyu.,Wu, Taihua.,Wang, Weidong.,...&Lin, Hongli.(2016).Development of a Functional Glomerulus at the Organ Level on a Chip to Mimic Hypertensive Nephropathy.SCIENTIFIC REPORTS,6. |
| MLA | Zhou, Mengying,et al."Development of a Functional Glomerulus at the Organ Level on a Chip to Mimic Hypertensive Nephropathy".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
来源：大连化学物理研究所
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