Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy
文献类型：期刊论文
| 作者 | Liu, Zheyi1,2; Wang, Fangjun2; Chen, Jin1,2; Zhou, Ye1,2; Zou, Hanfa2 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2016-08-26 |
| 卷号 | 1461页码:35-41 |
| 关键词 | Multi-phosphopeptide Enrichment Competitive Substitution Pre-coordination Mass Spectrometry Phosphoproteomics |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2016.07.042 |
| 文献子类 | Article |
| 英文摘要 | Although many affinity adsorbents have been developed for phosphopeptides enrichment, high specifically capturing the multi-phosphopeptides is still a big challenge. Here, we investigated the mechanism of phosphate ion coordination and substitution on affinity adsorbents surfaces and modulated the selectivity of affinity adsorbents to multi-phosphopeptides based on the different capability of mono and multi-phosphopeptides in competitively substituting the pre-coordinated phosphate ions at strong acidic condition. We demonstrated both the species of pre-coordinated phosphate ions and the substituting conditions played crucial roles in modulating the enrichment selectivity to multi-phosphopeptides, and the pre-coordinated affinity materials with relative more surfaces positive charges exhibited better enrichment efficiency due to the cooperative effect of electrostatic interaction and competitive substitution. Finally, an enrichment selectivity of 85% to multi-phosphopeptides was feasibly achieved with 66% improvement in identification numbers for complex protein sample extracted from HepG2 cells. Data are available via ProteomeXchange with identifier PXD004252. (c) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | PHOSPHORYLATED PEPTIDES ; PROTEIN-PHOSPHORYLATION ; MASS-SPECTROMETRY ; TITANIUM-DIOXIDE ; ENRICHMENT ; PHOSPHOPROTEOME ; CHROMATOGRAPHY ; SEPARATION ; NETWORKS ; TIO2 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000381840200005 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170300]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Wang, Fangjun |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zheyi,Wang, Fangjun,Chen, Jin,et al. Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1461:35-41. |
| APA | Liu, Zheyi,Wang, Fangjun,Chen, Jin,Zhou, Ye,&Zou, Hanfa.(2016).Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy.JOURNAL OF CHROMATOGRAPHY A,1461,35-41. |
| MLA | Liu, Zheyi,et al."Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy".JOURNAL OF CHROMATOGRAPHY A 1461(2016):35-41. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。