NiCoMnO4: A Bifunctional Affinity Probe for His-Tagged Protein Purification and Phosphorylation Sites Recognition
文献类型：期刊论文
| 作者 | Qi, Xiaoyue1; Chen, Long1; Zhang, Chaoqun2; Xu, Xinyuan1; Zhang, Yiding1; Bai, Yu1; Liu, Huwei1 |
| 刊名 | ACS Applied Materials & Interfaces
 |
| 出版日期 | 2016-07-27 |
| 卷号 | 8期号:29页码:18675-18683 |
| 关键词 | Affinity Probes His-tagged Proteins Purification Phosphopeptides Enrichment Metal Oxide Buffer Modulation |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.6b04280 |
| 文献子类 | Article |
| 英文摘要 | A bifunctional affinity probe NiCoMnO4 was designed and prepared with controllable morphology and size using facile methods. It was observed that the probe could be applied in His-tagged proteins purification and phosphopeptides enrichment simply through the buffer modulation. NiCoMnO4 particles showed satisfactory cycling performance for His-tagged proteins purification and broad pH-tolerance of loading buffer for phosphopeptides affinity. Therefore, a high-throughput, cost-effective, and efficient protein/peptide purification method was developed within 10 min based on the novel bifunctional affinity probe. |
| WOS关键词 | PHOSPHOPEPTIDE ENRICHMENT ; PHOSPHOPROTEOME ANALYSIS ; ELECTRICAL-PROPERTIES ; SELECTIVE ENRICHMENT ; MAGNETIC SEPARATION ; NANOPARTICLES ; NANOSPHERES ; ACTIVATION ; REGULATOR ; AUTOPHAGY |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000380735500006 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170326]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Bai, Yu; Liu, Huwei |
| 作者单位 | 1.Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci,Minist Educ, Beijing 100871, Peoples R China 2.Peking Univ, Beijing Nucl Magnet Resonance Ctr, Coll Life Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Xiaoyue,Chen, Long,Zhang, Chaoqun,et al. NiCoMnO4: A Bifunctional Affinity Probe for His-Tagged Protein Purification and Phosphorylation Sites Recognition[J]. ACS Applied Materials & Interfaces,2016,8(29):18675-18683. |
| APA | Qi, Xiaoyue.,Chen, Long.,Zhang, Chaoqun.,Xu, Xinyuan.,Zhang, Yiding.,...&Liu, Huwei.(2016).NiCoMnO4: A Bifunctional Affinity Probe for His-Tagged Protein Purification and Phosphorylation Sites Recognition.ACS Applied Materials & Interfaces,8(29),18675-18683. |
| MLA | Qi, Xiaoyue,et al."NiCoMnO4: A Bifunctional Affinity Probe for His-Tagged Protein Purification and Phosphorylation Sites Recognition".ACS Applied Materials & Interfaces 8.29(2016):18675-18683. |
入库方式： OAI收割
来源：大连化学物理研究所
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