Separation and characterization of bufadienolides in toad skin using two-dimensional normal-phase liquid chromatography x reversed-phase liquid chromatography coupled with mass spectrometry
文献类型：期刊论文
| 作者 | Zhang, Yun2; Jin, Hongli2; Li, Xiaolong2; Zhao, Jianqiang3; Guo, Xiujie2; Wang, Jixia2; Guo, Zhimou2; Zhang, Xiuli2; Tao, Yanduo3; Liu, Yanfang2 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2016-07-15 |
| 卷号 | 1026页码:67-74 |
| 关键词 | Bufadienolides Normal-phase Liquid Chromatography Two-dimensional Liquid Chromatography Ms/ms Toad Skin |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2015.11.015 |
| 文献子类 | Article |
| 英文摘要 | Bufadienolides possess various bioactivities especially antitumor. Due to the high structural diversity, the separation of bufadienolides often suffers from coelution problem on conventional RP columns. In this work, an off-line two-dimensional normal-phase liquid chromatography x reversed-phase liquid chromatography (2D-NPLC x RPLC) method was developed to separate and characterize bufadienolides in toad skin. Several RP and NP columns were evaluated with five reference bufadienlides. The XUnion C18 and XAmide columns exhibited superior chromatographic performances for bufadienlide separation, and were selected in RPLC and NPLC, respectively. RPLC was used in the second-dimension for the good compatibility with MS, while NPLC was adopted in the first-dimension. The orthogonality of the 2D-NPLC x RPLC system was investigated by the geometric approach using fifteen bufadienolide mixtures. The result was 49.6%, demonstrating reasonable orthogonality of this 2D-LC system. By combining the 2D-LC system with MS, 64 bufadienlides including 33 minor ones and 11 pairs of isomers in toad skin were identified. This off-line 2D-NPLC x RPLC allowed to solve the coelution problem of bufadienlides in one-dimension RPLC, and thus facilitated the identification significantly. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS关键词 | BUFO-GARGARIZANS CANTOR ; EFFICIENT PURIFICATION ; CARDIAC-GLYCOSIDES ; CHAN-SU ; ACTIVE BUFADIENOLIDES ; CELL-GROWTH ; HPLC-DAD ; STEROIDS ; CANCER ; IDENTIFICATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000379636800008 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170424]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Liu, Yanfang; Chen, Deliang |
| 作者单位 | 1.Wuhan Univ, Zhongnan Hosp, Dept Internal Med, Div Cardiol, Wuhan 430071, Hubei Province, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yun,Jin, Hongli,Li, Xiaolong,et al. Separation and characterization of bufadienolides in toad skin using two-dimensional normal-phase liquid chromatography x reversed-phase liquid chromatography coupled with mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1026:67-74. |
| APA | Zhang, Yun.,Jin, Hongli.,Li, Xiaolong.,Zhao, Jianqiang.,Guo, Xiujie.,...&Liang, Xinmiao.(2016).Separation and characterization of bufadienolides in toad skin using two-dimensional normal-phase liquid chromatography x reversed-phase liquid chromatography coupled with mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1026,67-74. |
| MLA | Zhang, Yun,et al."Separation and characterization of bufadienolides in toad skin using two-dimensional normal-phase liquid chromatography x reversed-phase liquid chromatography coupled with mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1026(2016):67-74. |
入库方式： OAI收割
来源：大连化学物理研究所
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