Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry
文献类型：期刊论文
| 作者 | Cheng, Likun1,2; Xu, Qingsong1; Yang, Kaiyun3; He, Jun3; Chen, Daiwen2; Du, Yuguang4; Yin, Heng1 |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2016-06-01 |
| 卷号 | 37期号:11页码:1525-1531 |
| 关键词 | Hydrophilic Interaction Chromatography Liquid Chromatography-mass Spectrometry Mass Spectrometry Porcine Milk Oligosaccharides |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.201500471 |
| 文献子类 | Article |
| 英文摘要 | Swine plays a significant role in livestock agriculture. As a linkage between sows and piglets, porcine milk is crucial for the health of newborn piglets. Free milk oligosaccharides (MOs) are kinds of important bioactive substance in mammalian milk. However, little is known about the component and function of the porcine MOs (PMOs). In this study, a hydrophilic interaction chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HILIC-Q-TOF MS) system was utilized to profile the PMOs. Forty-one distinct PMOs were identified totally in three breeds of sows. The PMOs were highly sialylated (similar to 30%) and fucosylated PMOs (1-3%) were monitored at low levels. The most abundant oligosaccharide was a trisaccharide (Hex(3)) which contributed over 50% of the total PMOs abundance. Comparison of free MOs profiles revealed heterogeneity and variations among individuals and different breeds of sows, however, the MOs variation among breeds was limited even minor than that among individuals. Furthermore, most PMOs contents were higher in colostrum and decreased in the early lactation, but a few kinds increased at last. Different oligosaccharides had different patterns during lactation. Overall, these observations showed a more detailed PMOs library and would contribute to the exploration of influence of PMOs on piglets' health. |
| WOS关键词 | BOVINE-MILK ; MICROBIOTA ; QUANTIFICATION ; GLYCANS ; GLYCOME |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000379135200015 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170460]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Yin, Heng |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Sichuan Agr Univ, Inst Anim Nutr, Chengdu, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, Likun,Xu, Qingsong,Yang, Kaiyun,et al. Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry[J]. ELECTROPHORESIS,2016,37(11):1525-1531. |
| APA | Cheng, Likun.,Xu, Qingsong.,Yang, Kaiyun.,He, Jun.,Chen, Daiwen.,...&Yin, Heng.(2016).Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry.ELECTROPHORESIS,37(11),1525-1531. |
| MLA | Cheng, Likun,et al."Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry".ELECTROPHORESIS 37.11(2016):1525-1531. |
入库方式： OAI收割
来源：大连化学物理研究所
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