Oxidized dextran facilitated synthesis of a silica-based concanavalin a material for lectin affinity enrichment of glycoproteins/glycopeptides
文献类型：期刊论文
| 作者 | Liu, Yujie1,2; Fu, Dongmei2; Yu, Long2,3; Xiao, Yuansheng2; Peng, Xiaojun1; Liang, Xinmiao2,3 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2016-07-15 |
| 卷号 | 1455页码:147-155 |
| 关键词 | Glycoprotein/glycopeptide Lectin Affinity Chromatography Mass Spectrometry Oxidized Dextran |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2016.05.093 |
| 文献子类 | Article |
| 英文摘要 | Lectin affinity chromatography (LAC) is an important enrichment technique in glycoproteomics analysis. In order to improve the effectiveness of enrichment, it is necessary to develop LAC materials with high specificity and efficiency. Herein, using oxidized dextran as the spacer, a silica-based concanavalin A material (SiO2-ODex Con A) was synthesized to enrich glycoproteins/glycopeptides. For comparison, the SiO2-Ald Con A material was synthesized using conventional (3-glycidoxypropyl) triethoxysilane (GPMS) as the initial spacer arm. The analytical merits of both Con A materials, such as non-specific adsorption, binding capacity and trapping efficiency, have been evaluated using ovalbumin. Under high performance liquid affinity chromatography (HPLAC) mode, the SiO2-ODex Con A material was highly effective in the enrichment of glycoproteins/glycopeptides attached to high-mannose-type and bi-antennary complex type glycans. The promising potential of the SiO2-ODex Con A material was demonstrated by selective fractionation of glycoproteins from complex biological samples for glycosylation analysis. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | MASS-SPECTROMETRIC ANALYSIS ; YEAST EXTERNAL INVERTASE ; GLYCOSYLATION SITES ; HUMAN SERUM ; CHROMATOGRAPHY ; GLYCOPROTEINS ; STRATEGIES ; OVALBUMIN ; SUPPORTS ; IDENTIFICATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000378954900017 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170462]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Yu, Long; Liang, Xinmiao |
| 作者单位 | 1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Nantong Univ, Coinnovat Ctr Neuroregenerat, Nantong 226019, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yujie,Fu, Dongmei,Yu, Long,et al. Oxidized dextran facilitated synthesis of a silica-based concanavalin a material for lectin affinity enrichment of glycoproteins/glycopeptides[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1455:147-155. |
| APA | Liu, Yujie,Fu, Dongmei,Yu, Long,Xiao, Yuansheng,Peng, Xiaojun,&Liang, Xinmiao.(2016).Oxidized dextran facilitated synthesis of a silica-based concanavalin a material for lectin affinity enrichment of glycoproteins/glycopeptides.JOURNAL OF CHROMATOGRAPHY A,1455,147-155. |
| MLA | Liu, Yujie,et al."Oxidized dextran facilitated synthesis of a silica-based concanavalin a material for lectin affinity enrichment of glycoproteins/glycopeptides".JOURNAL OF CHROMATOGRAPHY A 1455(2016):147-155. |
入库方式： OAI收割
来源：大连化学物理研究所
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