Further investigation of a peptide extraction method with mesoporous silica using high-performance liquid chromatography coupled with tandem mass spectrometry
文献类型：期刊论文
| 作者 | Du, Yan1,2; Wu, Dapeng1; Guan, Yafeng1 |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2016-06-01 |
| 卷号 | 39期号:11页码:2156-2163 |
| 关键词 | Extraction Mechanism Mesoporous Silica Peptide Extraction Serum |
| ISSN号 | 1615-9306 |
| DOI | 10.1002/jssc.201501354 |
| 文献子类 | Article |
| 英文摘要 | Mobil Composition of Matter No. 41 (MCM-41) was the most frequently used mesoporous silica material to extract peptides from complex biological samples. However, there were confusing extraction conditions and large extraction efficiency variance among related reports, which resulted from unclear understanding about the interaction between the material and peptides. In this study, the extraction mechanism was investigated with one set of tryptic peptides by using high-performance liquid chromatography coupled with triple quadrupole mass spectrometry. Generally, hydrophobic interaction and electrostatic attraction were two major driving forces for extraction of peptides, while electrostatic repulsion greatly weakened the interaction between the material and peptides with isoelectric points below the pH. With most peptides positively charged and MCM-41 slightly negatively charged, most efficient extraction was obtained at pH 3, and it was proved that electrostatic and hydrophobic interaction acted in synergy for extraction of all the peptides. A mixed solution of acetonitrile with buffers of high pH or ion strength was demonstrated to be favorable for elution, which performed much better than the commonly used eluate (mixture of acetonitrile with 0.1% trifluoroacetic acid). Finally, under optimum conditions, it was found that extraction efficiency of MCM-41 for protein digest and human serum was greatly improved. |
| WOS关键词 | SELECTIVE ENRICHMENT ; HUMAN PLASMA ; ENDOGENOUS PEPTIDES ; MOLECULAR-SIEVES ; FACILE SYNTHESIS ; MICROSPHERES ; ADSORPTION ; SEPARATION ; MS ; NANOPARTICLES |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000378680300017 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170490]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Wu, Dapeng; Guan, Yafeng |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Yan,Wu, Dapeng,Guan, Yafeng. Further investigation of a peptide extraction method with mesoporous silica using high-performance liquid chromatography coupled with tandem mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2016,39(11):2156-2163. |
| APA | Du, Yan,Wu, Dapeng,&Guan, Yafeng.(2016).Further investigation of a peptide extraction method with mesoporous silica using high-performance liquid chromatography coupled with tandem mass spectrometry.JOURNAL OF SEPARATION SCIENCE,39(11),2156-2163. |
| MLA | Du, Yan,et al."Further investigation of a peptide extraction method with mesoporous silica using high-performance liquid chromatography coupled with tandem mass spectrometry".JOURNAL OF SEPARATION SCIENCE 39.11(2016):2156-2163. |
入库方式： OAI收割
来源：大连化学物理研究所
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