Bioinspired Saccharide-Saccharide Interaction and Smart Polymer for Specific Enrichment of Sialylated Glycopeptides
文献类型：期刊论文
| 作者 | Li, Xiuling1; Xiong, Yuting2; Qing, Guangyan2; Jiang, Ge1; Li, Xianqin1; Sun, Taolei2; Liang, Xinmiao1 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2016-06-01 |
| 卷号 | 8期号:21页码:13294-13302 |
| 关键词 | Smart Polymer Saccharide Glycopeptide Sialic Acid Enrichment Hydrogen Bond |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.6b03104 |
| 文献子类 | Article |
| 英文摘要 | Abnormal sialylation of proteins is highly associated with many major diseases, such as cancers and neurodegenerative diseases. However, this study is challenging owing to the difficulty in enriching trace sialylated glycopeptides (SGs) from highly complex biosamples. The key to solving this problem relies strongly on the design of novel SG receptors to capture the sialic acid (SA) moieties in a specific and tunable manner. Inspired by the saccharide-saccharide interactions in life systems, here we introduce saccharide-based SG receptors into this study. Allose (a monosaccharide) displays specific and pH-sensitive binding toward SAs. Integrating allose units into a polyacrylamide chain generates a saccharide-responsive smart copolymer (SRSC). Such design significantly improves the selectivity of SA binding; meanwhile, this binding can be intelligently triggered in a large extent by solution polarity and pH. As a result, SRSC exhibits high-performance enrichment capacity toward SGs, even under 500-fold interference of bovine serum albumins digests, which is notably higher than conventional materials. In real biosamples of HeLa cell lysates, 180 sialylated glycosylation sites (SGSs) have been identified using SRSC. This is apparently superior to those obtained by SA-binding lectins including WGA (18 SGSs) and SNA (22 SGSs). Furthermore, lactose displays good chemoselectivity toward diverse disaccharides, which indicated the good potential of lactose-based material in glyean discrimination. Subsequently, the lactose-based SRSC facilitates the stepwise isolation of O-linked or N-linked SGs with the same peptide sequence but varied glycans by CH3CN/H2O gradients. This study opens a new avenue for next generation of glycopeptide enrichment materials. |
| WOS关键词 | HYDROPHILIC INTERACTION CHROMATOGRAPHY ; TITANIUM-DIOXIDE CHROMATOGRAPHY ; MASS-SPECTROMETRY ; SIALIC-ACID ; CARBOHYDRATE INTERACTION ; AFFINITY-CHROMATOGRAPHY ; GLYCOSYLATION ANALYSIS ; PROTEIN GLYCOSYLATION ; SELECTIVE ENRICHMENT ; ARTIFICIAL RECEPTORS |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000377150400013 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170568]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Qing, Guangyan; Sun, Taolei; Liang, Xinmiao |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, 122 Luoshi Rd, Wuhan 430070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xiuling,Xiong, Yuting,Qing, Guangyan,et al. Bioinspired Saccharide-Saccharide Interaction and Smart Polymer for Specific Enrichment of Sialylated Glycopeptides[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(21):13294-13302. |
| APA | Li, Xiuling.,Xiong, Yuting.,Qing, Guangyan.,Jiang, Ge.,Li, Xianqin.,...&Liang, Xinmiao.(2016).Bioinspired Saccharide-Saccharide Interaction and Smart Polymer for Specific Enrichment of Sialylated Glycopeptides.ACS APPLIED MATERIALS & INTERFACES,8(21),13294-13302. |
| MLA | Li, Xiuling,et al."Bioinspired Saccharide-Saccharide Interaction and Smart Polymer for Specific Enrichment of Sialylated Glycopeptides".ACS APPLIED MATERIALS & INTERFACES 8.21(2016):13294-13302. |
入库方式： OAI收割
来源：大连化学物理研究所
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