A highly selective near-infrared fluorescent probe for carboxylesterase 2 and its bioimaging applications in living cells and animals
文献类型：期刊论文
| 作者 | Jin, Qiang1,2; Feng, Lei3; Wang, Dan-Dan1; Wu, Jing-Jing1; Hou, Jie3,4; Dai, Zi-Ru1; Sun, Shi-Guo3; Wang, Jia-Yue1; Ge, Guang-Bo1,3; Cui, Jing-Nan3 |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2016-09-15 |
| 卷号 | 83页码:193-199 |
| 关键词 | Nir Fluorescent Probe Carboxylesterase 2 Enzymatic Activity Specific Detection Bioimaging |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2016.04.075 |
| 文献子类 | Article |
| 英文摘要 | A near-infrared fluorescent probe (DDAB) for highly selective and sensitive detection of carboxylesterase 2 (CE2) has been designed, synthesized, and systematically studied both in vitro and in vivo. Upon addition of CE2, the ester bond of DDAB could be rapidly cleaved and then release a near-infrared (NIR) fluorophore DDAO, which brings a remarkable yellow-to-blue color change and strong NIR fluorescence emission in physiological solutions. The newly developed probe exhibits excellent properties including good specificity, ultrahigh sensitivity and high imaging resolution. Moreover, DDAB has been applied to measure the real activities of CE2 in complex biological samples, as well as to screen CE2 inhibitors by using tissue preparations as the enzymes sources. The probe has also been successfully used to detect endogenous CE2 in living cells and in vivo for the first time, and the results demonstrate that such detection is highly reliable. All these prominent features of DDAB make it holds great promise for further investigation on CE2-associated biological process and for exploring the physiological functions of CE2 in living systems. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | IN-VIVO ; FAR-RED ; SMALL-INTESTINE ; HUMAN LIVER ; ACTIVATION ; EXPRESSION ; PRODRUG ; FLUOROPHORE ; METABOLISM ; IRINOTECAN |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000376802300027 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170598]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Ge, Guang-Bo; Cui, Jing-Nan; Yang, Ling |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China 4.Dalian Med Univ, Dalian 116044, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jin, Qiang,Feng, Lei,Wang, Dan-Dan,et al. A highly selective near-infrared fluorescent probe for carboxylesterase 2 and its bioimaging applications in living cells and animals[J]. BIOSENSORS & BIOELECTRONICS,2016,83:193-199. |
| APA | Jin, Qiang.,Feng, Lei.,Wang, Dan-Dan.,Wu, Jing-Jing.,Hou, Jie.,...&Yang, Ling.(2016).A highly selective near-infrared fluorescent probe for carboxylesterase 2 and its bioimaging applications in living cells and animals.BIOSENSORS & BIOELECTRONICS,83,193-199. |
| MLA | Jin, Qiang,et al."A highly selective near-infrared fluorescent probe for carboxylesterase 2 and its bioimaging applications in living cells and animals".BIOSENSORS & BIOELECTRONICS 83(2016):193-199. |
入库方式： OAI收割
来源：大连化学物理研究所
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