Detergent-Assisted Glycoprotein Capture: A Versatile Tool for In-Depth N-Glycoproteome Analysis
文献类型：期刊论文
| 作者 | Chen, Rui1,2; Zou, Hanfa3; Figeys, Daniel1,2 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2016-06-01 |
| 卷号 | 15期号:6页码:2080-2086 |
| 关键词 | Detergent Glycoprotein Capture Membrane Serum N-glycoproteome |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/acs.jproteome.6b00056 |
| 文献子类 | Article |
| 英文摘要 | Large-scale N-glycoproteome studies have been hindered by poor solubility of hydrophobic membrane proteins and the complexity of proteome samples. Herein, we developed a detergent-assisted glycoprotein capture method to reduce these issues by conducting hydrazide chemistry-based glycoprotein capture in the presence of strong detergents such as sodium dodecyl sulfate and Triton X-100. The strong detergents helped to solubilize hydrophobic membrane proteins and then increased the access of hydrazide groups to oxidized glycoproteins, thus increasing the coverage of the N-glycoproteome. Compared with the conventional glycopeptide capture method, the detergent-assisted glycoprotein capture approach nearly doubled the number of N-glycosylation sites identified from HEK 293T cells with improved specificity. Application of this approach in the larger scale N-glycoproteomics analysis of the HEK 293T cell membrane led to the identification of 2253 unique N-glycosites from 953 proteins. Furthermore, the application of this approach to human serum resulted in the identification of 850 N-glycosylation sites without any immunodepletion or fractionation. Overall, the detergent-assisted glycoprotein capture method simplified the capture process, and it increased the number of sites observed on both hydrophobic membrane proteins and hydrophilic secreted proteins. |
| WOS关键词 | HYDROPHILIC INTERACTION CHROMATOGRAPHY ; PROTEIN GLYCOSYLATION ; HYDRAZIDE CHEMISTRY ; MASS-SPECTROMETRY ; PLASMA PROTEOME ; IDENTIFICATION ; CANCER ; LIVER ; DISINTEGRIN ; PROTEOLYSIS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000377319200030 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170612]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Figeys, Daniel |
| 作者单位 | 1.Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa Inst Syst Biol, 451 Smyth Rd, Ottawa, ON K1H 8M5, Canada 2.Univ Ottawa, Dept Chem & Biomol Sci, 451 Smyth Rd, Ottawa, ON K1H 8M5, Canada 3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Rui,Zou, Hanfa,Figeys, Daniel. Detergent-Assisted Glycoprotein Capture: A Versatile Tool for In-Depth N-Glycoproteome Analysis[J]. JOURNAL OF PROTEOME RESEARCH,2016,15(6):2080-2086. |
| APA | Chen, Rui,Zou, Hanfa,&Figeys, Daniel.(2016).Detergent-Assisted Glycoprotein Capture: A Versatile Tool for In-Depth N-Glycoproteome Analysis.JOURNAL OF PROTEOME RESEARCH,15(6),2080-2086. |
| MLA | Chen, Rui,et al."Detergent-Assisted Glycoprotein Capture: A Versatile Tool for In-Depth N-Glycoproteome Analysis".JOURNAL OF PROTEOME RESEARCH 15.6(2016):2080-2086. |
入库方式： OAI收割
来源：大连化学物理研究所
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