Developing multi-cellular tumor spheroid model (MOTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening
文献类型：期刊论文
| 作者 | Chao, Ji-Ye2,3; Ruan, Wen-Ding2,3; Du, Feng-guang2,3; Meng, Yue-Zhong4; Wang, Duo2,3; Wang, Jian-Zheng1,2,3; Zhu, Yu-Xia1,2,3; Ma, Hui-Chao2,3; Chen, Si-Nan2,3 |
| 刊名 | MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
 |
| 出版日期 | 2016-05-01 |
| 卷号 | 62页码:215-225 |
| 关键词 | Three-dimensional Cell Culture Multi-cellular Tumor Spheroid (Mots) Spray-spinning Chitosan/collagen/alginate Scaffold (Cca) |
| ISSN号 | 0928-4931 |
| DOI | 10.1016/j.msec.2016.01.045 |
| 文献子类 | Article |
| 英文摘要 | In this work, a 3D MCTS-CCA system was constructed by culturing multi-cellular tumor spheroid (MCTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening. The CCA scaffolds were fabricated by spray-spinning. The interactions between the components of the spray-spun fibers were evidenced by methods of Coomassie Blue stain, X-ray diffraction (XRD) and Fourier transform-infrared spectroscopy (FTIR). Co-culture indicated that MCF-7 cells showed a spatial growth pattern of multi-cellular tumor spheroid (MCTS) in the CCA fibrous scaffold with increased proliferation rate and drug-resistance to MMC, ADM and 5-Aza comparing with the 2D culture cells. Significant increases of total viable cells were found in 3D MCTS groups after drug administration by method of apoptotic analysis. Glucose-lactate analysis indicated that the metabolism of MCTS in CCA scaffold was closer to the tumor issue in vivo than the monolayer cells. In addition, MCTS showed the characteristic of epithelial mesenchymal transition (EMT) which is subverted by carcinoma cells to facilitate metastatic spread. These results demonstrated that MCTS in CCA scaffold possessed a more conservative phenotype of tumor than monolayer cells, and anticancer drug screening in 3D MCTS-CCA system might be superior to the 2D culture system. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | MESENCHYMAL STEM-CELLS ; CANCER-CELL ; MULTICELLULAR SPHEROIDS ; CHITOSAN-ALGINATE ; MAMMALIAN TARGET ; EPITHELIAL-CELLS ; BREAST-CANCER ; MASS-TRANSFER ; CULTURE ; BONE |
| WOS研究方向 | Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000372759100027 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170642]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Wang, Jian-Zheng |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Tianguan Grp Co Ltd, Affiliated Gen Hosp, Nanyang 473000, Peoples R China 3.Testing Ctr Henan Tianguan Grp Co Ltd, 1 Tianguan Rd, Nanyang 473000, Henan Province, Peoples R China 4.Sun Yat Sen Univ, State Key Lab Optoelect Mat & Technol, Guangzhou 510275, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chao, Ji-Ye,Ruan, Wen-Ding,Du, Feng-guang,et al. Developing multi-cellular tumor spheroid model (MOTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2016,62:215-225. |
| APA | Chao, Ji-Ye.,Ruan, Wen-Ding.,Du, Feng-guang.,Meng, Yue-Zhong.,Wang, Duo.,...&Chen, Si-Nan.(2016).Developing multi-cellular tumor spheroid model (MOTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,62,215-225. |
| MLA | Chao, Ji-Ye,et al."Developing multi-cellular tumor spheroid model (MOTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 62(2016):215-225. |
入库方式： OAI收割
来源：大连化学物理研究所
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