Anti-gastric cancer activity in three-dimensional tumor spheroids of bufadienolides
文献类型：期刊论文
| 作者 | Zhang, Yun1; Hou, Tao1; Wei, Lai1; Wang, Jixia1; Zhang, Xiuli1,3; Li, Xiaolong1; Qu, Lala1; Shi, Liying1; Liu, Yanfang1; Zou, Lijuan2 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-04-21 |
| 卷号 | 6 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep24772 |
| 文献子类 | Article |
| 英文摘要 | Multicellular spheroids of cancer cells have been increasingly used to screen anti-tumor compounds, owing to their in vivo like microenvironment and structure as well as compatibility to high-throughput/high-content screening. Here we report the potency and efficacy of a family of bufadienolides to inhibit the growth of gastric cancer cell line HGC-27 in three-dimensional (3D) spheroidal models. Examining the morphological and growth patterns of several cell lines in round-bottomed ultra-low attachment microplate suggested that HGC-27 cells formed reproducibly multicellular spheroidal structures. Profiling of 15 natural bufadienolides isolated from toad skin indicated that 8 14-hydroxy bufadienolides displayed inhibitory activity of the growth of HGC-27 spheroids in a dose-dependent manner. Notably, compared to clinical drugs taxol and epirubicin, active bufadienolides were found to penetrate more effectively into the HGC-27 spheroids, but with a narrower effective concentration range and a shorter lasting inhibitory effect. Furthermore, compared to two-dimensional (2D) cell monolayer assays, active bufadienolides exhibited weaker efficacy and different potency in 3D spheroid model, demonstrating the great potential of 3D multicellular cell spheroid models in anti-cancer drug discovery and development. |
| WOS关键词 | BUFO-BUFO-GARGARIZANS ; BREAST-CANCER ; HEPATOCELLULAR-CARCINOMA ; CARDIAC-GLYCOSIDES ; PANCREATIC-CANCER ; DRUG-RESISTANCE ; CELL-CULTURE ; STEM-CELL ; 3D ; MODEL |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | NATURE PUBLISHING GROUP |
| WOS记录号 | WOS:000374453200001 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170770]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Xiuli; Liang, Xinmiao |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 2, Dept Radiat Oncol, Dalian 116027, Peoples R China 3.Nantong Univ, Coinnovat Ctr Neuroregenerat, Nantong 226019, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yun,Hou, Tao,Wei, Lai,et al. Anti-gastric cancer activity in three-dimensional tumor spheroids of bufadienolides[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Zhang, Yun.,Hou, Tao.,Wei, Lai.,Wang, Jixia.,Zhang, Xiuli.,...&Liang, Xinmiao.(2016).Anti-gastric cancer activity in three-dimensional tumor spheroids of bufadienolides.SCIENTIFIC REPORTS,6. |
| MLA | Zhang, Yun,et al."Anti-gastric cancer activity in three-dimensional tumor spheroids of bufadienolides".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
来源：大连化学物理研究所
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