Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6
文献类型：期刊论文
| 作者 | Bai, Fengwu1; Wang, Xiaohui1,2,3; Chi, Naiyu3; Du, Yuguang4; Zhao, Yong2; Yin, Heng2 |
| 刊名 | EXTREMOPHILES
 |
| 出版日期 | 2016-03-01 |
| 卷号 | 20期号:2页码:167-176 |
| 关键词 | Chitin Exochitinase Cold-adapted Salt-tolerant Pseudoalteromonas Sp. |
| ISSN号 | 1431-0651 |
| DOI | 10.1007/s00792-016-0810-5 |
| 文献子类 | Article |
| 英文摘要 | We have previously reported a non-processive endo-type chitinase, ChiA, from a newly isolated marine psychrophilic bacterium, Pseudoalteromonas sp. DL-6. In this study, a processive exo-type chitinase, ChiC, was cloned from the same bacterium and characterized in detail. ChiC could hydrolyze crystalline chitin into (GlcNAc)(2) as the only observed product. It exhibited high catalytic activity even at low temperatures, e.g. close to 0 degrees C, or in the presence of 5 M NaCl, suggesting that ChiC was a cold-adapted and highly salt-tolerant chitinase. ChiC could also hydrolyze other substrates, including chitosan and Avicel, indicating its broad substrate specificity. Sequence features indicated that ChiC was a multi-domain protein having a deep substrate-binding groove that was regarded as characteristic of processive exo-chitinases. Enzymatic hydrolysis of chitin by ChiC could be remarkably boosted in the presence of ChiA, suggesting the synergy of ChiC and ChiA. This work provided a new evidence to prove that marine psychrophilic bacteria utilized a synergistic enzyme system to degrade recalcitrant chitin. |
| WOS关键词 | MARINE PSYCHROPHILIC BACTERIUM ; SP STRAIN O-7 ; SERRATIA-MARCESCENS ; RECALCITRANT POLYSACCHARIDES ; BIOCHEMICAL-CHARACTERIZATION ; MORITELLA-MARINA ; BINDING DOMAIN ; BETA-CHITIN ; DEGRADATION ; PROCESSIVITY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000374832600006 |
| 出版者 | SPRINGER JAPAN KK |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170830]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhao, Yong; Yin, Heng |
| 作者单位 | 1.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Nat Prod & Glycobiotechnol Res Grp, Dalian 116023, Peoples R China 3.Dalian Univ, Sch Life Sci & Biotechnol, Dalian 116622, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Fengwu,Wang, Xiaohui,Chi, Naiyu,et al. Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6[J]. EXTREMOPHILES,2016,20(2):167-176. |
| APA | Bai, Fengwu,Wang, Xiaohui,Chi, Naiyu,Du, Yuguang,Zhao, Yong,&Yin, Heng.(2016).Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6.EXTREMOPHILES,20(2),167-176. |
| MLA | Bai, Fengwu,et al."Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6".EXTREMOPHILES 20.2(2016):167-176. |
入库方式： OAI收割
来源：大连化学物理研究所
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