A highly selective fluorescent probe for sensing activities of catechol-O-methyltransferase in complex biological samples
文献类型：期刊论文
| 作者 | Qian, Xing-Kai1,3; Wang, Ping3; Xia, Yang-Liu3; Dou, Tong-Yi3; Jin, Qiang3; Wang, Dan-Dan3; Hao, Da-Cheng2; Bi, Xiao-Lin1; Ge, Guang-Bo3; Yang, Ling3 |
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 231页码:615-623 |
| 关键词 | Catechol-o-methyltransferase Fluorescent Probe High-throughput Detection Bioimaging 7 8-dihydroxy-4-methylcoumarin |
| ISSN号 | 0925-4005 |
| DOI | 10.1016/j.snb.2016.03.074 |
| 文献子类 | Article |
| 英文摘要 | Catechol-O-methyltransferase (COMT), one of the most important phase II drug metabolizing enzymes, plays important roles in the metabolism of endogenous and xenobiotic catechols. In this study, a highly selective fluorescent probe for sensing activities of catechol-O-methyltransferase in complex biological samples was discovered and well characterized. Under physiological conditions, COMT selectively catalyzes the conversion of the probe (7,8-dihydroxy-4-methylcoumarin, DHMC) to 7-hydroxy-8methoxy-4-methylcoumarin (HMMC), which brings a strong turn-on fluorescence signal at 520 nm. The probe substrate has been used for monitoring the real activities of COMT in complex biological samples, as well as for rapid screening of potential COMT inhibitors which are useful in the treatment of Parkinson's diseases Furthermore, the probe has been successfully used to monitor endogenous COMT in living cells for the first time, and the results demonstrate that DHMC is cell membrane permeable and low toxic to the cells. All these features of DHMC suggested that this probe holds great promise for COMT-related regulation and inhibition assays in drug discovery, as well as for further investigation on the biological functions of COMT in living cells. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | METHYL TRANSFERASE ; FLUOROMETRIC-DETERMINATION ; CATALYTIC EFFICIENCY ; ASSAY ; INHIBITORS ; DISORDERS ; ESTROGENS ; ERYTHROCYTES ; SUBSTRATE ; DISEASE |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| WOS记录号 | WOS:000374330900070 |
| 出版者 | ELSEVIER SCIENCE SA |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170888]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Bi, Xiao-Lin; Ge, Guang-Bo; Yang, Ling |
| 作者单位 | 1.Dalian Med Univ, Inst Canc Stem Cell, Coll Basic Med Sci, Canc Ctr,Dept Biol Sci, Dalian 116044, Peoples R China 2.Dalian Jiaotong Univ, Dalian 116028, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qian, Xing-Kai,Wang, Ping,Xia, Yang-Liu,et al. A highly selective fluorescent probe for sensing activities of catechol-O-methyltransferase in complex biological samples[J]. SENSORS AND ACTUATORS B-CHEMICAL,2016,231:615-623. |
| APA | Qian, Xing-Kai.,Wang, Ping.,Xia, Yang-Liu.,Dou, Tong-Yi.,Jin, Qiang.,...&Yang, Ling.(2016).A highly selective fluorescent probe for sensing activities of catechol-O-methyltransferase in complex biological samples.SENSORS AND ACTUATORS B-CHEMICAL,231,615-623. |
| MLA | Qian, Xing-Kai,et al."A highly selective fluorescent probe for sensing activities of catechol-O-methyltransferase in complex biological samples".SENSORS AND ACTUATORS B-CHEMICAL 231(2016):615-623. |
入库方式： OAI收割
来源：大连化学物理研究所
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