Sepsis-induced impairment of neutrophil chemotaxis on a microfluidic chip
文献类型：期刊论文
| 作者 | Lu, Xiaoguang2; Lv, Chang3; Qu, Yueyang4; Luo, Yong4; Lin, Bingcheng1; Zhan, Libin5; Song, Yi2; Fan, Zhiwei2 |
| 刊名 | IMMUNOLOGY LETTERS
 |
| 出版日期 | 2016-05-01 |
| 卷号 | 173页码:55-60 |
| 关键词 | Neutrophil Chemotaxis Sepsis Tlr2 Cxcr2 Tlr2-cxcr2 Pathway Microfluidic Chip |
| ISSN号 | 0165-2478 |
| DOI | 10.1016/j.imlet.2016.03.010 |
| 文献子类 | Article |
| 英文摘要 | This study aimed to design a microfluidic chip to measure neutrophil chemotaxis, which is a convenient assay to assess the severity and prognosis of sepsis, and to study the mechanisms involved in the variation of neutrophil chemotaxis. Neutrophil chemotaxis was investigated in this microfluidic device by measuring the migration speed of neutrophils following the LPS concentration gradient stimulus. Neutrophils of 32 sepsis patients were divided into three groups according to the seriousness of physician-diagnosed sepsis, and 12 healthy individuals served as controls. Statistical significance was set at an alpha value of P<0.05. Neutrophil chemotaxis was significantly decreased following the seriousness of sepsis. By contrast, in septic neutrophils, the expression of TLR2 was significantly increased, whereas the expression of CXCR2 was significantly decreased. Neutrophil chemotaxis in sepsis was significantly reduced as compared to healthy individuals. We speculated that impaired neutrophil chemotaxis in sepsis was probably mediated by the TLR2-CXCR2 pathway. (C) 2016 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved. |
| WOS关键词 | STAPHYLOCOCCUS-AUREUS ; PLASMA-CONCENTRATIONS ; ORGAN FAILURE ; SEPTIC SHOCK ; MIGRATION ; DEPLETION ; LIPOPOLYSACCHARIDE ; RECEPTOR ; PROTEIN |
| WOS研究方向 | Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000375737800007 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170890]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Lu, Xiaoguang |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Liaoning, Peoples R China 2.Dalian Univ, Affiliated Zhongshan Hosp, Dept Emergency Med, 6 JieFang Ave, Dalian 116001, Liaoning, Peoples R China 3.Dalian Univ, Dept Med, Dalian 116622, Liaoning, Peoples R China 4.Dalian Univ Technol, Sch Pharmaceut Sci & Technol, Dalian 116024, Liaoning, Peoples R China 5.Nanjing Univ Chinese Med, Nanjing 210023, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Xiaoguang,Lv, Chang,Qu, Yueyang,et al. Sepsis-induced impairment of neutrophil chemotaxis on a microfluidic chip[J]. IMMUNOLOGY LETTERS,2016,173:55-60. |
| APA | Lu, Xiaoguang.,Lv, Chang.,Qu, Yueyang.,Luo, Yong.,Lin, Bingcheng.,...&Fan, Zhiwei.(2016).Sepsis-induced impairment of neutrophil chemotaxis on a microfluidic chip.IMMUNOLOGY LETTERS,173,55-60. |
| MLA | Lu, Xiaoguang,et al."Sepsis-induced impairment of neutrophil chemotaxis on a microfluidic chip".IMMUNOLOGY LETTERS 173(2016):55-60. |
入库方式： OAI收割
来源：大连化学物理研究所
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