Nonamplification Sandwich Assay Platform for Sensitive Nucleic Acid Detection Based on AuNPs Enumeration with the Dark-Field Microscope
文献类型：期刊论文
| 作者 | Zhang, Guoqing3; Li, Tian2; Xu, Xiao1; Lin, Ruoyun2; Chen, Yang2; Li, Chenxi2; Liu, Feng2; Li, Na2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2016-04-19 |
| 卷号 | 88期号:8页码:4188-4191 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.6b00535 |
| 文献子类 | Article |
| 英文摘要 | A simple and efficient assay platform with high sensitivity, convenient implementation, and moderate cost in reagents and instrumentation is most appropriate for routine applications. On the basis of the gold nanoparticle (AuNP) enumeration signal readout mode established in our laboratory, we have developed a nonamplification sandwich assay for nucleic acid detection with the 3 fM limit of detection for a sequence related to Alzheimer's disease. This AuNP counting based method takes advantages of the distinctive and strong localized surface plasmon resonance light scattering with the dark-field microscope and magnetic separation. It is shown that the presence of 20 nM random DNA sequence or calf thymus DNA with a mass up to 10(6)-fold of the targets do not significantly interfere with the assay signal. The spike recoveries of Hela cell lysate sample at 109.3% for 20 pM target and 110.5% for 100 pM target indicate the potential of this proposed method in practical sample applications. This nonamplification sandwich assay platform in principle is applicable to other assays such as the immunoassay and thus would be expected to find a breadth of applications that can make the best use of the simplicity and sensitivity. |
| WOS关键词 | SINGLE PLASMONIC NANOPARTICLES ; LIGATION CHAIN-REACTION ; GOLD NANOPARTICLES ; ULTRASENSITIVE DETECTION ; MESSENGER-RNA ; LIVING CELLS ; SCATTERING PROPERTIES ; HOMOGENEOUS DETECTION ; METAL NANOPARTICLES ; DNA HYBRIDIZATION |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000374706000005 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170896]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Li, Na |
| 作者单位 | 1.Natl Inst Metrol, Div Nano Metrol & Mat Measurement, Beijing 100029, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, Inst Analyt Chem,Minist Educ,BNLMS, Beijing 100871, Peoples R China 3.Suzhou Nanomicro Technol Co Ltd, Suzhou 215123, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Guoqing,Li, Tian,Xu, Xiao,et al. Nonamplification Sandwich Assay Platform for Sensitive Nucleic Acid Detection Based on AuNPs Enumeration with the Dark-Field Microscope[J]. ANALYTICAL CHEMISTRY,2016,88(8):4188-4191. |
| APA | Zhang, Guoqing.,Li, Tian.,Xu, Xiao.,Lin, Ruoyun.,Chen, Yang.,...&Li, Na.(2016).Nonamplification Sandwich Assay Platform for Sensitive Nucleic Acid Detection Based on AuNPs Enumeration with the Dark-Field Microscope.ANALYTICAL CHEMISTRY,88(8),4188-4191. |
| MLA | Zhang, Guoqing,et al."Nonamplification Sandwich Assay Platform for Sensitive Nucleic Acid Detection Based on AuNPs Enumeration with the Dark-Field Microscope".ANALYTICAL CHEMISTRY 88.8(2016):4188-4191. |
入库方式： OAI收割
来源：大连化学物理研究所
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