Aptamer functionalized hydrophilic polymer monolith with gold nanoparticles modification for the sensitive detection of human alpha-thrombin
文献类型：期刊论文
| 作者 | Chen, Yuanbo1,2; Deng, Nan1,2; Wu, Ci1,2; Liang, Yu1; Jiang, Bo1; Yang, Kaiguang1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | TALANTA
 |
| 出版日期 | 2016-07-01 |
| 卷号 | 154页码:555-559 |
| 关键词 | Aptamer Hydrophilic Monolith Human Alpha-thrombin Trace Protein Detection |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2016.02.054 |
| 文献子类 | Article |
| 英文摘要 | Low abundant proteins of body fluids participate nearly all physiological processes and indicate various kinds of diseases. The development of specific enrichment techniques is the key to identify and quantify the low abundant proteins. Herein, a novel kind of aptamer functionalized hydrophilic polymer monolith was developed for the specific enrichment and detection of human a -thrombin from the human plasma. Human a -thrombin aptamer, with thiol group modified at the 5' terminal, was immobilized on the gold nanoparticles (AuNPs) modified poly(glycidyl methacrylate-co-poly(ethylene glycol) diacrylate) monolithic column, with the binding capacity of 277.1 ilmol/L. Due to the hydrophilic poly(ethylene glycol) diacrylate) as the cross-linking monomer, the detection recovery of the aptamer-functionalized hydrophilic polymer monolithic column could reach to 92.6 +/- 5.2% (n=3) and the dynamic range could reach 0.5-300 ng/mu L (S/N > 10) with on-line UV detection. Meanwhile, the column could run over 100 times, because the poly(glycidyl methacrylate-co-poly(ethylene glycol) diacrylate) stability structure and the AuNPs improved the stability of the matrix material. Furthermore, this column could even capture the target alpha-thrombin, which was spiked in 1000 folds of original human plasma. All these results demonstrated the great potential of the prepared aptamer functionalized hydrophilic polymer monolith for the recognition of the trace proteins in the biological samples. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | IN-VITRO SELECTION ; AFFINITY-CHROMATOGRAPHY ; STATIONARY-PHASE ; CAPILLARY CHROMATOGRAPHY ; MAGNETIC BEADS ; ADENOSINE ; PROTEINS ; CAPTURE ; RECOGNITION ; EXTRACTION |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000376696400074 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170974]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Lihua |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Yuanbo,Deng, Nan,Wu, Ci,et al. Aptamer functionalized hydrophilic polymer monolith with gold nanoparticles modification for the sensitive detection of human alpha-thrombin[J]. TALANTA,2016,154:555-559. |
| APA | Chen, Yuanbo.,Deng, Nan.,Wu, Ci.,Liang, Yu.,Jiang, Bo.,...&Zhang, Yukui.(2016).Aptamer functionalized hydrophilic polymer monolith with gold nanoparticles modification for the sensitive detection of human alpha-thrombin.TALANTA,154,555-559. |
| MLA | Chen, Yuanbo,et al."Aptamer functionalized hydrophilic polymer monolith with gold nanoparticles modification for the sensitive detection of human alpha-thrombin".TALANTA 154(2016):555-559. |
入库方式： OAI收割
来源：大连化学物理研究所
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