Modulating fluorescence anisotropy of dye-labeled DNA without involving mass amplification
文献类型：期刊论文
| 作者 | Pei, Xiaojing; Huang, Hongduan; Chen, Yang; Li, Chenxi; Liu, Feng; Li, Na |
| 刊名 | TALANTA
 |
| 出版日期 | 2016-07-01 |
| 卷号 | 154页码:567-573 |
| 关键词 | Fluorescence Anisotropy Double-stranded Dna Leveling-off Phenomenon Middle-site-labeling c Overhang Mutiple Number Of Labels |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2016.01.016 |
| 文献子类 | Article |
| 英文摘要 | Fluorescence anisotropy, known as a simple, homogeneous and cost-effective analytical technology, is an invaluable technique for studying the micro -environmental changes of the dye associated with the molecular interactions. An in-depth understanding of the variables affecting the fluorescence anisotropy signal can facilitate better experimental designs to effectively improve the analytical performance. This work is a follow-up effort in evaluating the factors that can significantly influence fluorescence anisotropy. We systematically studied fluorescence anisotropy of dsDNA with the changing length based on dye-DNA interactions, with the fluorophores in the end -labeling, the middle -site -labeling, and multiple number of labeling manners. The fluorescence anisotropy value and the base -pair response dynamic range could be expanded by labeling the fluorophores in the middle of dsDNA and increasing the number of labels on dsDNA. The C overhang configuration in the end -labeling manner could enhance the fluorescence anisotropy signal but not expand the base -pair response range. Results from all the labeling fluorophores reinforced the leveling -off effect, i.e., the fluorescence anisotropy signal does not response to the increased length of the DNA duplex when the length is larger than a critical number of base pairs. These findings provide perspectives about choosing appropriate fluorescent dyes and labeling sites for simple and universal fluorescence anisotropy designs in various applications. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | SYBR-GREEN-I ; DOUBLE-STRANDED DNA ; SMALL MOLECULES ; SPECTROSCOPIC CHARACTERIZATION ; SENSITIVE DETECTION ; THIAZOLE ORANGE ; GROOVE-BINDING ; APTAMER ; POLARIZATION ; ASSAY |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000376696400076 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170982]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Li, Na |
| 作者单位 | Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn, BNLMS,Minist Educ,Inst Analyt Chem, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pei, Xiaojing,Huang, Hongduan,Chen, Yang,et al. Modulating fluorescence anisotropy of dye-labeled DNA without involving mass amplification[J]. TALANTA,2016,154:567-573. |
| APA | Pei, Xiaojing,Huang, Hongduan,Chen, Yang,Li, Chenxi,Liu, Feng,&Li, Na.(2016).Modulating fluorescence anisotropy of dye-labeled DNA without involving mass amplification.TALANTA,154,567-573. |
| MLA | Pei, Xiaojing,et al."Modulating fluorescence anisotropy of dye-labeled DNA without involving mass amplification".TALANTA 154(2016):567-573. |
入库方式： OAI收割
来源：大连化学物理研究所
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