Environment-induced conformational and functional changes of L-2-haloacid dehalogenase
文献类型：期刊论文
| 作者 | Wang, Yayue1,2; Cao, Xupeng1; Feng, Yanbin1; Xue, Song1 |
| 刊名 | JOURNAL OF BIOSCIENCE AND BIOENGINEERING
 |
| 出版日期 | 2016-05-01 |
| 卷号 | 121期号:5页码:491-496 |
| 关键词 | L-2-haloacid Dehalogenase Circular Dichroism Environmental Effects Conformation |
| ISSN号 | 1389-1723 |
| DOI | 10.1016/j.jbiosc.2015.09.008 |
| 文献子类 | Article |
| 英文摘要 | 2-Haloacid dehalogenases have been highly studied due to their potential applications in chemical industries and bioremediation. Although biochemical and structural characterizations of the enzyme have been detailed, no information was available regarding environmental effects on the structure function relationship. Here, circular dichroism spectroscopy (CD) was used to investigate the correlation between changes on the conformation and the function of L-2-haloacid dehalogenase (HadL AJ1) from the Pseudomonas putida induced by the environmental factors. Decreased alpha-helix and increased beta-sheet contents were observed in the structure of HadL AJ1 along with activity losses caused by pH, temperature and inhibitors. Regardless of which factor above-mentioned existed, more than 65.0% of HadL AJ1 activity could be remained if its alpha-helix content was over 12.0%. The maintenance of alpha-helical structure in HadL All was indispensable to its catalysis, while beta-sheet increase restricts its activity. This study revealed the variation of enzymatic activity due to environmental conditions resulting in structural changes monitored by CD, which contributed to rational modification and was instructive for predicting changes of the enzymatic activity during application. (C) 2015, The Society for Biotechnology, Japan. All rights reserved. |
| WOS关键词 | BACTERIAL 2-HALOACID DEHALOGENASES ; XANTHOBACTER-AUTOTROPHICUS GJ10 ; SPONGE HYMENIACIDON-PERLEVIS ; BURKHOLDERIA-CEPACIA MBA4 ; PSEUDOMONAS SP. YL ; HALOACID DEHALOGENASE ; CIRCULAR-DICHROISM ; ACID DEHALOGENASE ; CRYSTAL-STRUCTURE ; THERMAL-DENATURATION |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000376695300003 |
| 出版者 | SOC BIOSCIENCE BIOENGINEERING JAPAN |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/170992]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Xue, Song |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Sch Chem & Chem Engn, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yayue,Cao, Xupeng,Feng, Yanbin,et al. Environment-induced conformational and functional changes of L-2-haloacid dehalogenase[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2016,121(5):491-496. |
| APA | Wang, Yayue,Cao, Xupeng,Feng, Yanbin,&Xue, Song.(2016).Environment-induced conformational and functional changes of L-2-haloacid dehalogenase.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,121(5),491-496. |
| MLA | Wang, Yayue,et al."Environment-induced conformational and functional changes of L-2-haloacid dehalogenase".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 121.5(2016):491-496. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。