Probing the G-quadruplex from hsa-miR-3620-5p and inhibition of its interaction with the target sequence
文献类型：期刊论文
| 作者 | Tan, Wei1; Zhou, Jiang1; Gu, Jiangyong1; Xu, Ming2; Xu, Xiaojie1; Yuan, Gu1 |
| 刊名 | TALANTA
 |
| 出版日期 | 2016-07-01 |
| 卷号 | 154页码:560-566 |
| 关键词 | Microrna G-quadruplex Folding Pattern Natural Alkaloid Esi-ms |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2016.02.037 |
| 文献子类 | Article |
| 英文摘要 | G-quadruplexes have been reported to exist both in human genome and transcriptome and are of great interests due to their important biological functions. Up to now, the formation and property of G-quadruplex in human mature microRNAs has not been explored yet. In this study, we discovered that hsa-miR-3620-5p, a guanine rich human mature microRNA, could fold into a stable parallel G-quadruplex in near physiological condition for the first time. We explored the formation, folding pattern and binding affinity of the miR-3620-5p G-quadruplex by ESI-MS, CD, NMR and SPR. The results indicated that its high -order structure was comprised of three G-quartets with two bases in each parallel loop stretching outward and two bases flanking at each end. In addition, sanguinarine, a natural alkaloid screened from traditional Chinese medicine was characterized to have high binding affinity and thermodynamic stabilization effects through 7c -n stacking interaction with the external G-quartets. Furthermore, the potent interaction of sanguinarine with miR-3620-5p G-quadruplex could block the base pairing between miR-3620-5p and its target sequence. Therefore, our study revealed the possibility of regulating microRNA functions using potent G-quadruplex binders, and could provide a new approach to affect the microRNA:mRNA interactions. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | MICRORNA BIOGENESIS ; RNA QUADRUPLEX ; DNA ; BIOMARKER ; DISEASE ; CANCERS ; BINDING ; GENOME ; ROLES ; CELLS |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000376696400075 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171002]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhou, Jiang; Yuan, Gu |
| 作者单位 | 1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Dept Chem Biol,Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Peking Univ, Hosp 3, Dept Cardiol, Inst Vasc Med, Beijing 100191, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tan, Wei,Zhou, Jiang,Gu, Jiangyong,et al. Probing the G-quadruplex from hsa-miR-3620-5p and inhibition of its interaction with the target sequence[J]. TALANTA,2016,154:560-566. |
| APA | Tan, Wei,Zhou, Jiang,Gu, Jiangyong,Xu, Ming,Xu, Xiaojie,&Yuan, Gu.(2016).Probing the G-quadruplex from hsa-miR-3620-5p and inhibition of its interaction with the target sequence.TALANTA,154,560-566. |
| MLA | Tan, Wei,et al."Probing the G-quadruplex from hsa-miR-3620-5p and inhibition of its interaction with the target sequence".TALANTA 154(2016):560-566. |
入库方式： OAI收割
来源：大连化学物理研究所
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