Design of Organic Vapor Assisted Tight Ion Source and Its Application in Trace Analysis of Proteomic Samples
文献类型：期刊论文
| 作者 | Zhu Xu-Dong1,2; Zhou Yuan1; Liang Zhen1; Liang Yu1; Zhang Li-Hua1; Zhu Yi-Xin3; Zhang Yu-Kui1 |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2015-10-01 |
| 卷号 | 43期号:10页码:1433-1438 |
| 关键词 | Coanda Effect Electrospray Ionization Source Proteome Trace Analysis |
| ISSN号 | 0253-3820 |
| 文献子类 | Article |
| 英文摘要 | A novel Coanda effect electrospray ionization source (CEESI) was designed and applied in the analysis of the digests of bovine serum albumin (BSA) and proteins extracted from HeLa cells. Firstly, the effects of various organic solvents on the mass spectrometry signal of a standard peptide were investigated. With ethanol vapor, the signal of standard peptide was increased by about 1000 fold, and the sequence coverage of 1 ng of BSA was improved by about 10%. Finally, under the optimized conditions, 6, 10, 20 and 50 ng of HeLa cell tryptic digests were qualitatively analyzed with nano. HPLC. CEESI. LTQ XL MS system. In comparison with commercial nano. ESI, the number of identified peptides and proteins were increased by 20% - 360% and 20% - 200%, respectively, demonstrating that the CEESI ion source is promising in the in. depth profiling of trace proteomic samples. |
| WOS关键词 | ELECTROSPRAY DROPLET EXPOSURE ; CHARGE-STATE DISTRIBUTIONS ; CHROMATOGRAPHY-MASS SPECTROMETRY ; IONIZATION ; MECHANISM ; MANIPULATION |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000371688100002 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171034]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhu Xu-Dong |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Zhejiang Haochuang Biotech Co Ltd, Hangzhou 311121, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu Xu-Dong,Zhou Yuan,Liang Zhen,et al. Design of Organic Vapor Assisted Tight Ion Source and Its Application in Trace Analysis of Proteomic Samples[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2015,43(10):1433-1438. |
| APA | Zhu Xu-Dong.,Zhou Yuan.,Liang Zhen.,Liang Yu.,Zhang Li-Hua.,...&Zhang Yu-Kui.(2015).Design of Organic Vapor Assisted Tight Ion Source and Its Application in Trace Analysis of Proteomic Samples.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,43(10),1433-1438. |
| MLA | Zhu Xu-Dong,et al."Design of Organic Vapor Assisted Tight Ion Source and Its Application in Trace Analysis of Proteomic Samples".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 43.10(2015):1433-1438. |
入库方式： OAI收割
来源：大连化学物理研究所
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