Induction of predominant tenogenic phenotype in human dermal fibroblasts via synergistic effect of TGF-beta and elongated cell shape
文献类型：期刊论文
| 作者 | Wang, Wenbo1; Li, Jie1; Wang, Keyun3,4; Zhang, Zhiyong1,2; Zhang, Wenjie1,2; Zhou, Guangdong1,2; Cao, Yilin1,2; Ye, Mingliang3,4; Zou, Hanfa3,4; Liu, Wei1,2 |
| 刊名 | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
 |
| 出版日期 | 2016-03-01 |
| 卷号 | 310期号:5页码:C357-C372 |
| 关键词 | Tenogenic Phenotype Cell Elongation Dermal Fibroblasts Tgf-beta Rock Activity |
| ISSN号 | 0363-6143 |
| DOI | 10.1152/ajpcell.00300.2015 |
| 文献子类 | Article |
| 英文摘要 | Micropattern topography is widely investigated for its role in mediating stem cell differentiation, but remains unexplored for phenotype switch between mature cell types. This study investigated the potential of inducing tenogenic phenotype in human dermal fibroblasts (hDFs) by artificial elongation of cultured cells. Our results showed that a parallel microgrooved topography could convert spread hDFs into an elongated shape and induce a predominant tenogenic phenotype as the expression of biomarkers was significantly enhanced, such as scleraxis, tenomodulin, collagens I, III, VI, and decorin. It also enhanced the expression of transforming growth factor (TGF)-beta 1, but not alpha-smooth muscle actin. Elongated hDFs failed to induce other phenotypes, such as adiopogenic, chondrogenic, neurogenic, and myogenic lineages. By contrast, no tenogenic phenotype could be induced in elongated human chondrocytes, although chondrogenic phenotype was inhibited. Exogenous TGF-beta 1 could enhance the tenogenic phenotype in elongated hDFs at low dose (2 ng/ml), but promoted myofibroblast transdifferentiation of hDFs at high dose (10 ng/ml), regardless of cell shape. Elongated shape also resulted in decreased RhoA activity and increased Rho-associated protein kinase (ROCK) activity. Antagonizing TGF-beta or inhibiting ROCK activity with Y27632 or depolymerizing actin with cytochalasin D could all significantly inhibit tenogenic phenotype induction, particularly in elongated hDFs. In conclusion, elongation of cultured dermal fibroblasts can induce a predominant tenogenic phenotype likely via synergistic effect of TGF-beta and cytoskeletal signaling. |
| WOS关键词 | MESENCHYMAL STEM-CELLS ; CRUCIATE LIGAMENT CELLS ; CYTOSKELETAL TENSION ; EPITHELIAL-CELLS ; GENE-EXPRESSION ; IN-VITRO ; DIFFERENTIATION ; TENDON ; NUCLEUS ; TISSUE |
| WOS研究方向 | Cell Biology ; Physiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000371299500004 |
| 出版者 | AMER PHYSIOLOGICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171038]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Liu, Wei |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Shanghai Key Lab Tissue Engn, Dept Plast & Reconstruct Surg,Sch Med, Shanghai 200011, Peoples R China 2.Natl Tissue Engn Ctr China, Shanghai, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Analyt Chem, Natl Chromatog R&A Ctr, Dalian, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Wenbo,Li, Jie,Wang, Keyun,et al. Induction of predominant tenogenic phenotype in human dermal fibroblasts via synergistic effect of TGF-beta and elongated cell shape[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,2016,310(5):C357-C372. |
| APA | Wang, Wenbo.,Li, Jie.,Wang, Keyun.,Zhang, Zhiyong.,Zhang, Wenjie.,...&Liu, Wei.(2016).Induction of predominant tenogenic phenotype in human dermal fibroblasts via synergistic effect of TGF-beta and elongated cell shape.AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,310(5),C357-C372. |
| MLA | Wang, Wenbo,et al."Induction of predominant tenogenic phenotype in human dermal fibroblasts via synergistic effect of TGF-beta and elongated cell shape".AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY 310.5(2016):C357-C372. |
入库方式： OAI收割
来源：大连化学物理研究所
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