Cloning and evaluation of different constitutive promoters in the oleaginous yeast Rhodosporidium toruloides
文献类型：期刊论文
| 作者 | Wang, Yanan1,3; Lin, Xinping2; Zhang, Sufang1; Sun, Wenyi1; Ma, Sijia1; Zhao, Zongbao Kent1 |
| 刊名 | YEAST
 |
| 出版日期 | 2016-03-01 |
| 卷号 | 33期号:3页码:99-106 |
| 关键词 | Rhodosporidium Toruloides Promoter Transformation Genetic Engineering |
| ISSN号 | 0749-503X |
| DOI | 10.1002/yea.3145 |
| 文献子类 | Article |
| 英文摘要 | The oleaginous yeast Rhodosporidium toruloides is an unconventional yeast species that can accumulate a high content of lipids. Because it belongs to the basidiomycetous group of fungus, limited tools and functional elements are available for genetic engineering of R. toruloides and related red yeasts. Here we report the functional evaluation of five constitutive promoters from this yeast. We assembled a reporter gene expression cassette, consisting of a promoter, the hygromycin gene (HYG) and the nos terminator, and inserted it into the binary vector pZPK. Hygromycin-resistant transformants were obtained when R. toruloides cells were co-cultured with Agrobacterium tumefaciens AGL1 cells harbouring the engineered vector. Genomic integration of the reporter cassette was verified by successful amplification of target DNA fragments. Quantitative PCR analysis suggested that the transformant had only one copy of the reporter cassette. The strength of these promoters was demonstrated at the phenotypic level on the hygromycin-gradient plate and at the transcriptional level by real-time quantitative PCR. It was found that the strengths of these promoters varied no more than five-fold and followed a decreasing sequence of PPGI, PPGK, PFBA, PTPI, and PGPD. This study established new genetic elements for the construction of superior R. toruloides strains to produce advanced biofuels and related chemicals. Copyright (C) 2015 John Wiley & Sons, Ltd. |
| WOS关键词 | SACCHAROMYCES-CEREVISIAE ; LIPID PRODUCTION ; GENE-EXPRESSION ; MULTIPLE GENES ; TRANSFORMATION ; AGROBACTERIUM ; VECTORS ; LIBRARY ; GENOME |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology |
| 语种 | 英语 |
| WOS记录号 | WOS:000371935200003 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171052]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Sufang |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 2.Dalian Polytech Univ, Sch Food Sci & Technol, Dalian 116034, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yanan,Lin, Xinping,Zhang, Sufang,et al. Cloning and evaluation of different constitutive promoters in the oleaginous yeast Rhodosporidium toruloides[J]. YEAST,2016,33(3):99-106. |
| APA | Wang, Yanan,Lin, Xinping,Zhang, Sufang,Sun, Wenyi,Ma, Sijia,&Zhao, Zongbao Kent.(2016).Cloning and evaluation of different constitutive promoters in the oleaginous yeast Rhodosporidium toruloides.YEAST,33(3),99-106. |
| MLA | Wang, Yanan,et al."Cloning and evaluation of different constitutive promoters in the oleaginous yeast Rhodosporidium toruloides".YEAST 33.3(2016):99-106. |
入库方式： OAI收割
来源：大连化学物理研究所
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