Optimization of large-scale pseudotargeted metabolomics method based on liquid chromatography-mass spectrometry
文献类型：期刊论文
| 作者 | Luo, Ping1; Yin, Peiyuan1; Zhang, Weijian2; Zhou, Lina1; Lu, Xin1; Lin, Xiaohui2; Xu, Guowang1 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2016-03-11 |
| 卷号 | 1437页码:127-136 |
| 关键词 | Pseudotargeted Analysis Metabolomics Metabolic Profiling Lc-ms Quality Control Large-scale |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2016.01.078 |
| 文献子类 | Article |
| 英文摘要 | Liquid chromatography-mass spectrometry (LC-MS) is now a main stream technique for large-scale metabolic phenotyping to obtain a better understanding of genomic functions. However, repeatability is still an essential issue for the LC-MS based methods, and convincing strategies for long time analysis are urgently required. Our former reported pseudotargeted method which combines nontargeted and targeted analyses, is proved to be a practical approach with high-quality and information-rich data. In this study, we developed a comprehensive strategy based on the pseudotargeted analysis by integrating blank-wash, pooled quality control (QC) sample, and post-calibration for the large-scale metabolomics study. The performance of strategy was optimized from both pre- and post-acquisition sections including the selection of QC samples, insertion frequency of QC samples, and post-calibration methods. These results imply that the pseudotargeted method is rather stable and suitable for large-scale study of metabolic profiling. As a proof of concept, the proposed strategy was applied to the combination of 3 independent batches within a time span of 5 weeks, and generated about 54% of the features with coefficient of variations (CV) below 15%. Moreover, the stability and maximal capability of a single analytical batch could be extended to at least 282 injections (about 110 h) while still providing excellent stability, the CV of 63% metabolic features was less than 15%. Taken together, the improved repeatability of our strategy provides a reliable protocol for large-scale metabolomics studies. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | GENOME-WIDE ASSOCIATION ; TARGETED METABOLOMICS ; BIOMARKER DISCOVERY ; GAS-CHROMATOGRAPHY ; METABOLITE LEVELS ; HUMAN BLOOD ; HUMAN SERUM ; UPLC-MS ; NORMALIZATION ; PERFORMANCE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000371556700014 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171060]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Xu, Guowang |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Dalian Univ Technol, Sch Comp Sci & Technol, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, Ping,Yin, Peiyuan,Zhang, Weijian,et al. Optimization of large-scale pseudotargeted metabolomics method based on liquid chromatography-mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1437:127-136. |
| APA | Luo, Ping.,Yin, Peiyuan.,Zhang, Weijian.,Zhou, Lina.,Lu, Xin.,...&Xu, Guowang.(2016).Optimization of large-scale pseudotargeted metabolomics method based on liquid chromatography-mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1437,127-136. |
| MLA | Luo, Ping,et al."Optimization of large-scale pseudotargeted metabolomics method based on liquid chromatography-mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1437(2016):127-136. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。