A Two-Photon Ratiometric Fluorescent Probe for Imaging Carboxylesterase 2 in Living Cells and Tissues
文献类型：期刊论文
| 作者 | Ge, Guang-Bo1,3; Jin, Qiang1; Feng, Lei3; Wang, Dan-Dan1; Dai, Zi-Ru1; Wang, Ping1; Zou, Li-Wei1; Liu, Zhi-Hong2; Wang, Jia-Yue1; Yu, Yang1 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2015-12-30 |
| 卷号 | 7期号:51页码:28474-28481 |
| 关键词 | Two-photon Ratiometric Fluorescent Probe Carboxylesterase 2 Specific Sensing Bioimaging |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.5b09573 |
| 文献子类 | Article |
| 英文摘要 | In this study, a two-photon ratiometric fluorescent probe NCEN has been designed and developed for highly selective and sensitive sensing of human carboxylesterase 2 (hCE2) based on the catalytic properties and substrate preference of hCE2. Upon addition of hCE2, the probe could be readily hydrolyzed to release 4-amino-1,8-naphthalimide (NAH), which brings remarkable red-shift in fluorescence (90 nm) spectrum. The newly developed probe exhibits good specificity, ultrahigh sensitivity, and has been successfully applied to determine the real activities of hCE2 in complex biological samples such as cell and tissue preparations. NCEN has also been used for two-photon imaging of intracellular hCE2 in living cells as well as in deep-tissues for the first time, and the results showed that the probe exhibited high ratiometric imaging resolution and deep-tissue imaging depth. All these findings suggested that this probe holds great promise for applications in bioimaging of endogenous hCE2 in living cells and in exploring the biological functions of hCE2 in complex biological systems. |
| WOS关键词 | LIVER CARBOXYLESTERASE ; SMALL-INTESTINE ; TUMOR-TISSUE ; MICROSCOPY ; PRODRUG ; HYDROLYSIS ; ACTIVATION ; CAPECITABINE ; MITOCHONDRIA ; IRINOTECAN |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000369448200058 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171180]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Ge, Guang-Bo |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Wuhan Univ, Coll Chem & Mol Sci, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Peoples R China 3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Guang-Bo,Jin, Qiang,Feng, Lei,et al. A Two-Photon Ratiometric Fluorescent Probe for Imaging Carboxylesterase 2 in Living Cells and Tissues[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(51):28474-28481. |
| APA | Ge, Guang-Bo.,Jin, Qiang.,Feng, Lei.,Wang, Dan-Dan.,Dai, Zi-Ru.,...&Yang, Ling.(2015).A Two-Photon Ratiometric Fluorescent Probe for Imaging Carboxylesterase 2 in Living Cells and Tissues.ACS APPLIED MATERIALS & INTERFACES,7(51),28474-28481. |
| MLA | Ge, Guang-Bo,et al."A Two-Photon Ratiometric Fluorescent Probe for Imaging Carboxylesterase 2 in Living Cells and Tissues".ACS APPLIED MATERIALS & INTERFACES 7.51(2015):28474-28481. |
入库方式： OAI收割
来源：大连化学物理研究所
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