Manual-slide-engaged paper chip for parallel SERS-immunoassay measurement of clenbuterol from swine hair
文献类型：期刊论文
| 作者 | Lin, Bingcheng3; Zheng, Tingting1,2; Gao, Zhigang1,2; Luo, Yong1,2; Liu, Xianming1,3; Zhao, Weijie1,2 |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2016-02-01 |
| 卷号 | 37期号:3页码:418-424 |
| 关键词 | Clenbuterol Hair Analysis Paper Microfluidics Sers |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.201500324 |
| 文献子类 | Article |
| 英文摘要 | Clenbuterol (CL), as a feed additive, has been banned in many countries due to its potential threat to human health. In detection of CL, a fast, low-cost technique with high accuracy and specificity would be ideal for its administrative on-field inspections. Among the attempts to pursue a reliable detection tool of CL, a technique that combines surface enhanced Raman spectroscopy (SERS) and immunoassay, is close to meet the requirements as above. However, multiple steps of interactions between CL analyte, antibody, and antigen are involved in this method, and under conventional setup, the operation of SERS/immunoassay were unwieldy. In this paper, to facilitate a more manageable sample manipulation for SERS-immunoassay measurement, a 3D paper chip was suggested. A switch-on-chip multilayered (abbreviated as SoCM-) microfluidic paper-based analysis device (Pad) was fabricated to provide operators with manual switches on the interactions between different microfluids. Besides, on a detection slip we made on the main body of our SoCM-Pad, antigen was anchored in pattern. With this architecture, multistep interactions between the CL analyte in swine hair extract and the SERS probe-modified antibody and antigen, were managed for on-chip SERS-immunoassay detection. This would be very attractive for fast, cheap, accurate, and on-site specific detection of CL from real samples. |
| WOS关键词 | ENHANCED RAMAN-SPECTROSCOPY ; BETA-AGONISTS ; CAPILLARY-ELECTROPHORESIS ; MICROFLUIDIC PAPER ; RAPID-DETERMINATION ; ENZYME-IMMUNOASSAY ; MASS-SPECTROMETRY ; URINE ; ELECTROCHEMILUMINESCENCE ; INSTRUMENTATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000369959400006 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171182]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Luo, Yong; Liu, Xianming |
| 作者单位 | 1.Dalian Univ Technol, Dept Chem Engn, State Key Lab Fine Chem, Dalian, Peoples R China 2.Dalian Univ Technol, Sch Pharmaceut Sci & Technol, Dalian 116012, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Bingcheng,Zheng, Tingting,Gao, Zhigang,et al. Manual-slide-engaged paper chip for parallel SERS-immunoassay measurement of clenbuterol from swine hair[J]. ELECTROPHORESIS,2016,37(3):418-424. |
| APA | Lin, Bingcheng,Zheng, Tingting,Gao, Zhigang,Luo, Yong,Liu, Xianming,&Zhao, Weijie.(2016).Manual-slide-engaged paper chip for parallel SERS-immunoassay measurement of clenbuterol from swine hair.ELECTROPHORESIS,37(3),418-424. |
| MLA | Lin, Bingcheng,et al."Manual-slide-engaged paper chip for parallel SERS-immunoassay measurement of clenbuterol from swine hair".ELECTROPHORESIS 37.3(2016):418-424. |
入库方式： OAI收割
来源：大连化学物理研究所
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