Culture of low density E. coli cells in alginate chitosan microcapsules facilitates stress resistance by up-regulating luxS/AI-2 system
文献类型：期刊论文
| 作者 | Zheng, Huizhen1,2; Gao, Meng1,2; Song, Huiyi1,2; Ren, Ying1,2; Li, Shen1,2; Liu, Xiudong3; Yu, Weiting2; Ma, Xiaojun2 |
| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2016-05-05 |
| 卷号 | 141页码:160-165 |
| 关键词 | Alginate-chitosan Microencapsulation Entrapped Low-density Cells With Culture Quorum Sensing Viability |
| ISSN号 | 0144-8617 |
| DOI | 10.1016/j.carbpol.2016.01.011 |
| 文献子类 | Article |
| 英文摘要 | Entrapped low density cells with culture (ELDCwc) have been proved as a more effective way than direct entrapped high density cells (dEHDC) and free cells to protect probiotics from harsh environment, that is, to improve their stress resistance. The aim of this study was to investigate whether bacterial quorum sensing (QS) facilitated the stress resistance of Escherichia coli in microcapsules by detecting the expression of luxS/AI-2 system. As a result, both the expression of luxS gene and the concentration of autoinducer-2 (AI-2, QS signal molecule) have been discovered higher in ELDCwc than in dEHDC and free cells. Besides that, the luxS mutant E. coli strain was used as a negative control of QS to verify the influence of QS on bacterial stress resistance in microcapsules. The significantly decreased viability of luxS mutant strain in simulated gastric fluid also indicated that the QS played a critical role in protecting microorganisms from severe environment. (C) 2016 Elsevier Ltd. All rights reserved. |
| WOS关键词 | LACTOBACILLUS-RHAMNOSUS ; ESCHERICHIA-COLI ; SALMONELLA-TYPHIMURIUM ; MICROENCAPSULATION ; SURVIVAL ; PROBIOTICS ; MICROENVIRONMENT ; THERMOTOLERANCE ; ENCAPSULATION ; CEREVISIAE |
| WOS研究方向 | Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000369960400019 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171216]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Liu, Xiudong; Yu, Weiting |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 3.Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Huizhen,Gao, Meng,Song, Huiyi,et al. Culture of low density E. coli cells in alginate chitosan microcapsules facilitates stress resistance by up-regulating luxS/AI-2 system[J]. CARBOHYDRATE POLYMERS,2016,141:160-165. |
| APA | Zheng, Huizhen.,Gao, Meng.,Song, Huiyi.,Ren, Ying.,Li, Shen.,...&Ma, Xiaojun.(2016).Culture of low density E. coli cells in alginate chitosan microcapsules facilitates stress resistance by up-regulating luxS/AI-2 system.CARBOHYDRATE POLYMERS,141,160-165. |
| MLA | Zheng, Huizhen,et al."Culture of low density E. coli cells in alginate chitosan microcapsules facilitates stress resistance by up-regulating luxS/AI-2 system".CARBOHYDRATE POLYMERS 141(2016):160-165. |
入库方式： OAI收割
来源：大连化学物理研究所
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