Identification of Characteristic Fatty Acids to Quantify Triacylglycerols in Microalgae
文献类型：期刊论文
| 作者 | Shen, Pei-Li1,4; Wang, Hai-Tao1,4; Pan, Yan-Fei3; Meng, Ying-Ying2; Wu, Pei-Chun1; Xue, Song1 |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2016-02-22 |
| 卷号 | 7 |
| ISSN号 | 1664-462X |
| 关键词 | Microalgae Triacylglycerols Neutral Lipids Characteristic Fattyacids Biofuels |
| DOI | 10.3389/fpls.2016.00162 |
| 文献子类 | Article |
| 英文摘要 | The fatty acid profiles of lipids from microalgae are unique. Polyunsaturated fatty acids are generally enriched in polar lipids, whereas saturated and monounsaturated fatty acids constitute the majority of fatty acids in triacylglycerols (TAG). Each species has characteristic fatty acids, and their content is positively or negatively correlated with TAGs. The marine oleaginous diatom Phaeodactylum tricornutum was used as the paradigm to determine the quantitative relationship between TAG and characteristic fatty acid content. Fatty acid profiles and TAG content of Phaeodactylum tricornutum were determined in a time course. C16:0/C16:1 and eicosapentaenoic acid (EPA, C20:5n3) were identified as characteristic fatty acids in TAGs and polar lipids, respectively. The percentage of those characteristic fatty acids in total fatty acids had a significant linear relationship with TAG content, and thus, the correlation coefficient presenting r(2) were 0.96, 0.94, and 0.97, respectively. The fatty acid-based method for TAG quantification could also be applied to other microalgae such as Nannochloropsis oceanica in which the r(2) of C16:0 and EPA were 0.94 and 0.97, respectively, and in Chlorella pyrenoidosa-values for C18:1 and C18:3 with TAG content were 0.91 and 0.99, repectively. This characteristic fatty acid-based method provided a distinct way to quantify TAGs in microalgae, by which TAGs could be measured precisely by immediate transesterification from wet biomass rather than using conventional methods. This procedure simplified the operation and required smaller samples than conventional methods. |
| WOS关键词 | CHLAMYDOMONAS-REINHARDTII ; NANNOCHLOROPSIS-OCEANICA ; NITROGEN STARVATION ; ARACHIDONIC-ACID ; ACCUMULATION ; BIODIESEL ; LIPIDS ; DESATURASE |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000370543500001 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171246]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Xue, Song |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Marine Bioengn Grp, Dalian, Peoples R China 2.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian, Peoples R China 3.Dalian Univ, Coll Environm & Chem Engn, Dept Environm Sci & Engn, Dalian 116012, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Pei-Li,Wang, Hai-Tao,Pan, Yan-Fei,et al. Identification of Characteristic Fatty Acids to Quantify Triacylglycerols in Microalgae[J]. FRONTIERS IN PLANT SCIENCE,2016,7. |
| APA | Shen, Pei-Li,Wang, Hai-Tao,Pan, Yan-Fei,Meng, Ying-Ying,Wu, Pei-Chun,&Xue, Song.(2016).Identification of Characteristic Fatty Acids to Quantify Triacylglycerols in Microalgae.FRONTIERS IN PLANT SCIENCE,7. |
| MLA | Shen, Pei-Li,et al."Identification of Characteristic Fatty Acids to Quantify Triacylglycerols in Microalgae".FRONTIERS IN PLANT SCIENCE 7(2016). |
入库方式： OAI收割
来源：大连化学物理研究所
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