Quantitative analysis of protein orientation in membrane environments by kinase activity
文献类型：期刊论文
| 作者 | Liu, Baoquan1,2,3; Fan, Shengdi1,2,3; Xiong, Wen1,2,3; Quan, Chunshan1,2,3; Zhang, Xuning4; Wang, Lina5; Jin, Liming1,2,3 |
| 刊名 | JOURNAL OF BIOSCIENCE AND BIOENGINEERING
 |
| 出版日期 | 2016-02-01 |
| 卷号 | 121期号:2页码:242-246 |
| 关键词 | Agrc Protein Orientation Liposome Thiol-reactive Reagent Kinase Activity |
| ISSN号 | 1389-1723 |
| DOI | 10.1016/j.jbiosc.2015.06.002 |
| 文献子类 | Article |
| 英文摘要 | AgrC is an integral membrane receptor protein with histidine kinase activity in the accessory gene regulator (agr) quorum-sensing system of Staphylococcus aureus. In this study, proteoliposomes were used as a model to investigate AgrC orientation. Many approaches have been described to determine membrane protein orientation, but they are often complicated and time consuming. In this study, AgrC orientation in liposomes was determined by thiol-reactive reagent labeling and a kinase activity assay. Our results suggest use of a kinase activity assay could get an accurate percentage of functional protein orientation and only cost nearly one-sixth of the time compared with the method based on thiol-reactive reagent labeling. We present an effective and rapid method for determining the orientation of membrane protein kinases like AgrC. (C) 2015, The Society for Biotechnology, Japan. All rights reserved. |
| WOS关键词 | STAPHYLOCOCCUS-AUREUS ; RECONSTITUTION ; SOLUBILIZATION ; LIPOSOMES ; AGRC ; INFECTIONS ; DETERGENTS ; VIRULENCE ; MIXTURES ; RECEPTOR |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000370887300018 |
| 出版者 | SOC BIOSCIENCE BIOENGINEERING JAPAN |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171266]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Quan, Chunshan |
| 作者单位 | 1.Dalian Nationalities Univ, Econ & Technol Dev Zone, Minist Educ, Dalian 116600, Peoples R China 2.Dalian Nationalities Univ, Dept Life Sci, Econ & Technol Dev Zone, Dalian 116600, Peoples R China 3.Dalian Nationalities Univ, Key Lab Biotechnol & Resource Utilizat, State Ethn Affairs Commiss, Econ & Technol Dev Zone, Dalian 116600, Peoples R China 4.Dalian Polytechn Univ, Sch Biol Engn, 1 Qinggongyuan, Dalian 116034, Peoples R China 5.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhong Shan Rd, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Baoquan,Fan, Shengdi,Xiong, Wen,et al. Quantitative analysis of protein orientation in membrane environments by kinase activity[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2016,121(2):242-246. |
| APA | Liu, Baoquan.,Fan, Shengdi.,Xiong, Wen.,Quan, Chunshan.,Zhang, Xuning.,...&Jin, Liming.(2016).Quantitative analysis of protein orientation in membrane environments by kinase activity.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,121(2),242-246. |
| MLA | Liu, Baoquan,et al."Quantitative analysis of protein orientation in membrane environments by kinase activity".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 121.2(2016):242-246. |
入库方式： OAI收割
来源：大连化学物理研究所
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