Determination of Internal Controls for Quantitative Gene Expression of Isochrysis zhangjiangensis at Nitrogen Stress Condition
文献类型：期刊论文
| 作者 | Zhou Jiannan; Cao Xupeng; Wu Shuang; Xue Song |
| 刊名 | JOURNAL OF OCEAN UNIVERSITY OF CHINA
 |
| 出版日期 | 2016-02-01 |
| 卷号 | 15期号:1页码:137-144 |
| 关键词 | Chrysophyta Isochrysis Zhangjiangensis Nitrogen Deficiency Lipid Accumulation Reference Gene Selection |
| ISSN号 | 1672-5182 |
| DOI | 10.1007/s11802-016-2847-6 |
| 文献子类 | Article |
| 英文摘要 | Isochrysis zhangjiangensis is a potential marine microalga for biodiesel production, which accumulates lipid under nitrogen limitation conditions, but the mechanism on molecular level is veiled. Quantitative real-time polymerase chain reaction (qPCR) provides the possibility to investigate the gene expression levels, and a valid reference for data normalization is an essential prerequisite for firing up the analysis. In this study, five housekeeping genes, actin (ACT), alpha-tubulin (TUA), beta-tubulin (TUB), ubiquitin (UBI), 18S rRNA (18S) and one target gene, diacylglycerol acyltransferase (DGAT), were used for determining the reference. By analyzing the stabilities based on calculation of the stability index and on operating the two types of software, geNorm and bestkeeper, it showed that the reference genes widely used in higher plant and microalgae, such as UBI, TUA and 18S, were not the most stable ones in nitrogen-stressed I. zhangjiangensis, and thus are not suitable for exploring the mRNA expression levels under these experimental conditions. Our results show that ACT together with TUB is the most feasible internal control for investigating gene expression under nitrogen-stressed conditions. Our findings will contribute not only to future qPCR studies of I. zhangjiangensis, but also to verification of comparative transcriptomics studies of the microalgae under similar conditions. |
| WOS关键词 | REAL-TIME PCR ; MESSENGER-RNA ; RT-PCR ; CHLAMYDOMONAS-REINHARDTII ; HOUSEKEEPING GENES ; NITRATE TRANSPORTER ; TRANSCRIPT LEVELS ; QUANTIFICATION ; NORMALIZATION ; TRIACYLGLYCEROLS |
| WOS研究方向 | Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:000371073400017 |
| 出版者 | OCEAN UNIV CHINA |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171276]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Xue Song |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou Jiannan,Cao Xupeng,Wu Shuang,et al. Determination of Internal Controls for Quantitative Gene Expression of Isochrysis zhangjiangensis at Nitrogen Stress Condition[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2016,15(1):137-144. |
| APA | Zhou Jiannan,Cao Xupeng,Wu Shuang,&Xue Song.(2016).Determination of Internal Controls for Quantitative Gene Expression of Isochrysis zhangjiangensis at Nitrogen Stress Condition.JOURNAL OF OCEAN UNIVERSITY OF CHINA,15(1),137-144. |
| MLA | Zhou Jiannan,et al."Determination of Internal Controls for Quantitative Gene Expression of Isochrysis zhangjiangensis at Nitrogen Stress Condition".JOURNAL OF OCEAN UNIVERSITY OF CHINA 15.1(2016):137-144. |
入库方式： OAI收割
来源：大连化学物理研究所
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