Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells
文献类型：期刊论文
| 作者 | Lian, Jianchun3,4; Lv, Shijie1; Liu, Chang3,5; Liu, Yang3; Wang, Shujun3; Guo, Xin3; Nan, Feng2; Yu, Hua1; He, Xin4; Sun, Guangwei3 |
| 刊名 | STEM CELLS INTERNATIONAL
 |
| 出版日期 | 2016 |
| ISSN号 | 1687-966X |
| DOI | 10.1155/2016/9291013 |
| 文献子类 | Article |
| 英文摘要 | Background and Objective. It is important to guarantee the quality of stem cells. Serial passage is the main approach to expand stem cells. This study evaluated effects of serial passage on the biological characteristics of human umbilical cord Wharton's jelly-derived MSCs (WJ MSCs). Methods. Biological properties of WJ MSCs in the early (less than 10 passages, P10), middle (P11-20), and late (more than P20) phases including cell proliferation, cell cycle, phenotype, senescence, oncogene expression, stemness marker expression, and differentiation capacity were evaluated using flow cytometry, real-time PCR, immunocytofluorescence, and western blot. Results. It was found that there were no significant differences in cell proliferation, cell cycle, phenotype, and stemness marker expression in different phases. However, the expression of senescence-related gene, p21, and oncogene, c-Myc, was significantly upregulated in the late phase, which had close relations with the obviously increased cell senescence. Moreover, cardiac differentiation capability of WJ MSCs decreased whereas the propensity for neural differentiation increased significantly in the middle phase. Conclusions. This study reveals that WJ MSCs in the early and middle phases are relatively stable, and effect of serial passage on the lineage-specific differentiation should be considered carefully. |
| WOS关键词 | BONE-MARROW ; GENE-EXPRESSION ; PROLIFERATION ; ACTIVATION ; PATHWAY ; ADIPOSE |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000369722900001 |
| 出版者 | HINDAWI PUBLISHING CORP |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171362]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Liu, Yang |
| 作者单位 | 1.Dalian Matern & Child Healthcare Hosp, Dalian 116033, Peoples R China 2.Dalian Med Univ, Hosp 2, Dept Orthoped, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biotechnol, Dalian 116023, Peoples R China 4.Dalian Med Univ, Lab Med Coll, Dalian 116044, Peoples R China 5.Uni Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lian, Jianchun,Lv, Shijie,Liu, Chang,et al. Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells[J]. STEM CELLS INTERNATIONAL,2016. |
| APA | Lian, Jianchun.,Lv, Shijie.,Liu, Chang.,Liu, Yang.,Wang, Shujun.,...&Ma, Xiaojun.(2016).Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells.STEM CELLS INTERNATIONAL. |
| MLA | Lian, Jianchun,et al."Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells".STEM CELLS INTERNATIONAL (2016). |
入库方式： OAI收割
来源：大连化学物理研究所
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