Screening and identification of DPP-IV inhibitory peptides from deer skin hydrolysates by an integrated approach of LC-MS/MS and in silico analysis
文献类型：期刊论文
| 作者 | Jin, Yan1; Yan, Jiaze1; Yu, Yang1,2; Qi, Yanxia2 |
| 刊名 | JOURNAL OF FUNCTIONAL FOODS
 |
| 出版日期 | 2015-10-01 |
| 卷号 | 18页码:344-357 |
| 关键词 | Dipeptidyl Peptidase Iv Inhibitors Bioactive Peptides Liquid Chromatography And tAndem Mass Spectrometry (Lc-ms/ms) Deer Skin Collagen Integrated Approach |
| ISSN号 | 1756-4646 |
| DOI | 10.1016/j.jff.2015.07.015 |
| 文献子类 | Article |
| 英文摘要 | An integrated approach combining liquid chromatography/tandem mass spectrometry (LC-MS/MS) with in silico analysis was used to screen dipeptidyl peptidase IV (DPP-IV) inhibitory peptides from deer skin hydrolysates. A total of 203, 244 and 60 peptides were identified from deer skin hydrolysates prepared with pepsin, pepsin + trypsin and pepsin + Alcalase, respectively, by LC-MS/MS. The percentages of peptides in the above mentioned hydrolysates containing a Pro residue at the penultimate position were 5.9, 20.9 and 20.0%, respectively. Five peptides with a Pro residue at the penultimate position were synthesized and assessed, and these five synthetic peptides possessed DPP-IV inhibitory activity with IC50 values from 83.3 to 1638.3 mu M. One of the evaluated peptides contained an oxidized methionine. The effects of peptide modification and length on the DPP-IV inhibitory activity of the peptides were assessed. The results suggest that the integrated approach was efficient in identifying novel bioactive peptides from hydrolysates. (C) 2015 Elsevier Ltd. All rights reserved. |
| WOS关键词 | PROTEIN-DERIVED PEPTIDES ; INDUCED DIABETIC-RATS ; GELATIN HYDROLYSATE ; COLLAGEN HYDROLYSATE ; BIOACTIVE PEPTIDES ; GLYCEMIC CONTROL ; MASS-SPECTROMETRY ; DIETARY PROTEINS ; ENZYME ; ANTIOXIDANT |
| WOS研究方向 | Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000367757400032 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171452]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Jin, Yan |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Dalian Ocean Univ, Dalian 116623, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jin, Yan,Yan, Jiaze,Yu, Yang,et al. Screening and identification of DPP-IV inhibitory peptides from deer skin hydrolysates by an integrated approach of LC-MS/MS and in silico analysis[J]. JOURNAL OF FUNCTIONAL FOODS,2015,18:344-357. |
| APA | Jin, Yan,Yan, Jiaze,Yu, Yang,&Qi, Yanxia.(2015).Screening and identification of DPP-IV inhibitory peptides from deer skin hydrolysates by an integrated approach of LC-MS/MS and in silico analysis.JOURNAL OF FUNCTIONAL FOODS,18,344-357. |
| MLA | Jin, Yan,et al."Screening and identification of DPP-IV inhibitory peptides from deer skin hydrolysates by an integrated approach of LC-MS/MS and in silico analysis".JOURNAL OF FUNCTIONAL FOODS 18(2015):344-357. |
入库方式： OAI收割
来源：大连化学物理研究所
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