Stirring rate regulates the proliferation and metabolism of microencapsulated recombinant CHO cells
文献类型：期刊论文
| 作者 | Wang, Yu1,2; Zhang, Ying1; Li, Na1,2; Chen, Li1,2; Zhang, Demeng1,2; Sun, Dongsheng1,2; Li, Shen1,2; Guo, Xin1; Ma, Xiaojun1 |
| 刊名 | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
 |
| 出版日期 | 2015-11-01 |
| 卷号 | 62期号:6页码:833-839 |
| 关键词 | Cell Culture Microencapsulated Recombinant Protein Expression Stirring Rate |
| ISSN号 | 0885-4513 |
| DOI | 10.1002/bab.1333 |
| 文献子类 | Article |
| 英文摘要 | Stirred tank bioreactors are the most widely used method for the large-scale culture of mammalian cells. However, the scale of stirred tank bioreactors is limited by insufficient oxygen/nutrient mixing and the accumulation of waste products in high cell density cultures. The most effective method to solve these problems is to increase the stirring rate; this usually leads to increased cell proliferation, but can decrease the utilization of nutrients for recombinant protein synthesis. To investigate the effects of stirring rate on the proliferation, metabolism, and recombinant protein yield of microencapsulated recombinant Chinese hamster ovary (rCHO) cells, the cells were cultured under different stirring rates, and cell viability, metabolic activity, and protein yield were measured. Microencapsulation promoted Desmodus rotundus salivary plasminogen activator expression, and higher stirring rates promoted increases in microencapsulated cell density and metabolic activity. However, the maximum yield of recombinant protein was obtained at a moderate stirring rate, whereas protein yield was decreased at the highest tested stirring rate. The stirring rate had a significant impact on the growth and protein expression of microencapsulated rCHO cells, and a specific stirring rate was identified to maximize the yield of recombinant protein. (C) 2014 International Union of Biochemistry and Molecular Biology, Inc. |
| WOS关键词 | CULTURE ; TEMPERATURE ; PLASMINOGEN ; GROWTH |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000368361300013 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171476]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Ying |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yu,Zhang, Ying,Li, Na,et al. Stirring rate regulates the proliferation and metabolism of microencapsulated recombinant CHO cells[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2015,62(6):833-839. |
| APA | Wang, Yu.,Zhang, Ying.,Li, Na.,Chen, Li.,Zhang, Demeng.,...&Ma, Xiaojun.(2015).Stirring rate regulates the proliferation and metabolism of microencapsulated recombinant CHO cells.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,62(6),833-839. |
| MLA | Wang, Yu,et al."Stirring rate regulates the proliferation and metabolism of microencapsulated recombinant CHO cells".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 62.6(2015):833-839. |
入库方式： OAI收割
来源：大连化学物理研究所
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