Fabrication of a tunable hydrogel membrane for constructing indirect cell coculture system
文献类型：期刊论文
| 作者 | Song, Yizhe2,3; Zheng, Guoshuang2,3; Zhang, Demeng2,3; Lv, Yan2,3; Li, Na2,3; Wang, Xiuli1; Yu, Weiting2; Ma, Xiaojun2 |
| 刊名 | JOURNAL OF APPLIED POLYMER SCIENCE
 |
| 出版日期 | 2016-03-20 |
| 卷号 | 133期号:12 |
| 关键词 | Biomedical Applications Gels Membranes |
| ISSN号 | 0021-8995 |
| DOI | 10.1002/app.43100 |
| 文献子类 | Article |
| 英文摘要 | In this study, an improved indirect cell coculture system was constructed by using a polyelectrolyte complex membrane generated by alginate (A) and chitosan (C). Methodologies of characterizing thickness and permeability of flat AC membrane were first established due to the importance of these two parameters in determining intercellular distance and degree of contact between cocultured cells. Compared to reaction time, both alginate concentration and molecular weight (M-w) of chitosan play more dominant roles in determining the membrane thickness and diffusion coefficients. Moreover, cells in the alginate gel and on the AC membrane could maintain high cell viability. Thus, an improved indirect cell coculture system constructed by flat AC membrane was fabricated and characterized, which provides a robust tool to study the effect of intercellular distance and degree of contact between cocultured cells on cell-cell interactions. (C) 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43100. |
| WOS关键词 | EMBRYONIC STEM-CELLS ; ALGINATE GEL BEADS ; EXTERNAL GELATION ; POROUS MEMBRANES ; MICROCAPSULES ; CULTURE ; CHITOSAN ; PROLIFERATION ; HEPATOCYTES ; TECHNOLOGY |
| WOS研究方向 | Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000367913500008 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171504]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Wang, Xiuli |
| 作者单位 | 1.Dalian Med Univ, Coll Basic Med Sci, Dept Histol & Embryol, Dalian 116044, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Yizhe,Zheng, Guoshuang,Zhang, Demeng,et al. Fabrication of a tunable hydrogel membrane for constructing indirect cell coculture system[J]. JOURNAL OF APPLIED POLYMER SCIENCE,2016,133(12). |
| APA | Song, Yizhe.,Zheng, Guoshuang.,Zhang, Demeng.,Lv, Yan.,Li, Na.,...&Ma, Xiaojun.(2016).Fabrication of a tunable hydrogel membrane for constructing indirect cell coculture system.JOURNAL OF APPLIED POLYMER SCIENCE,133(12). |
| MLA | Song, Yizhe,et al."Fabrication of a tunable hydrogel membrane for constructing indirect cell coculture system".JOURNAL OF APPLIED POLYMER SCIENCE 133.12(2016). |
入库方式： OAI收割
来源：大连化学物理研究所
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