Characterization of a new family 75 chitosanase from Aspergillus sp W-2
文献类型：期刊论文
| 作者 | Zhang, Jianping1,3; Cao, Hailong3; Li, Shuguang3; Zhao, Yong3; Wang, Wenxia3; Xu, Qingsong3; Du, Yuguang2,3; Yin, Heng3 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2015-11-01 |
| 卷号 | 81页码:362-369 |
| 关键词 | Chitosanase ASp.rgillus Sp. Glycoside Hydrolyase Family 75 |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2015.08.026 |
| 文献子类 | Article |
| 英文摘要 | A new chitosanase gene of glycoside hydrolase (GH) family 75, csnw2, was cloned from an isolated strain Aspergillus sp. W-2 (CGMCC7018). The mature CsnW2 protein, fused to His-tag at C-terminus, was expressed in Pichia pastoris X-33 and purified with the affinity chromatography of Ni2+-NTA. The novel recombinant CsnW2 showed maximal activity with chitosan at pH 6.0 and 55 degrees C. Moreover, it had good pH stability and thermostability at a broad pH range of 3.0-10.0 and a temperature range of 30-70 degrees C, respectively. The enzymatic activity of the CsnW2 could be significantly enhanced by Ca2+, Mn2+ and Mg2+ at a concentration of 1 mM, but strongly inhibited by Fe2+, Zn2+, Ge2+, Ni2+ and Cu2+ above 1 mM. The CsnW2 showed specific hydrolytic activity against chitosan and preferred to hydrolyze chitosan with high degree of deacetylation. The main products of chtiosan (92% deacetylation) were chitosan oligosaccharides (COS) with degree of polymerization (DP) arranging from 2 to 6. Combined with the hydrolysis of COS from DP2 to DP6, CsnW2 was considered to be an endo-acting chitosanase. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS关键词 | OLIGOSACCHARIDES ; FUMIGATUS ; CHITOOLIGOSACCHARIDES ; MECHANISM ; CLONING ; CHITIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000367408300044 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171510]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Cao, Hailong |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Beijing 10090, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jianping,Cao, Hailong,Li, Shuguang,et al. Characterization of a new family 75 chitosanase from Aspergillus sp W-2[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,81:362-369. |
| APA | Zhang, Jianping.,Cao, Hailong.,Li, Shuguang.,Zhao, Yong.,Wang, Wenxia.,...&Yin, Heng.(2015).Characterization of a new family 75 chitosanase from Aspergillus sp W-2.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,81,362-369. |
| MLA | Zhang, Jianping,et al."Characterization of a new family 75 chitosanase from Aspergillus sp W-2".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 81(2015):362-369. |
入库方式： OAI收割
来源：大连化学物理研究所
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